This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Abe, H.
Right arrow Articles by Sasakawa, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Abe, H.
Right arrow Articles by Sasakawa, C.

 Previous Article  |  Next Article 

Infection and Immunity, July 2002, p. 3500-3509, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3500-3509.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Bicarbonate Ion Stimulates the Expression of Locus of Enterocyte Effacement-Encoded Genes in Enterohemorrhagic Escherichia coli O157:H7

Hiroyuki Abe,1 Ichiro Tatsuno,1 Toru Tobe,1 Akiko Okutani,2 and Chihiro Sasakawa1*

Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639,1 Laboratory of Veterinary Public Health, Graduate School of Agriculture and Life Science, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan2

Received 13 December 2001/ Returned for modification 6 February 2002/ Accepted 17 March 2002

Enterohemorrhagic Escherichia coli (EHEC) strains adhere to the intestinal mucosa and produce an attaching and effacing (A/E) lesion. Most of the genes required to produce A/E lesions are thought to be encoded by the 36-kb pathogenicity island termed the locus for enterocyte effacement (LEE). Although the mechanisms underlying the bacterial adherence, including the genes involved, are still poorly understood, the preferential adherence phenotype of EHEC is thought to depend on the nature of the genes and/or the response of these genes to changes in environmental conditions. To explore the environmental factors affecting EHEC adherence, we used an O157:H7 strain and investigated the optimal growth conditions for its adherence to Caco-2 cells. We observed that EHEC grown in Dulbecco's modified Eagle's medium (DMEM) adhered more efficiently to Caco-2 cells than EHEC grown in Luria-Bertani (LB) broth. Among the components of DMEM, only NaHCO3 was found to remarkably stimulate bacterial adherence. When bacteria were grown in LB broth containing NaHCO3, the production of intimin, Tir, EspA, and EspB was greatly enhanced compared with the production in LB broth. Indeed, the transcription of ler required for LEE-encoded gene expression was promoted in response to the concentration of NaHCO3 in LB broth. Since the concentration of NaHCO3 in the lower intestinal tract has been shown to be relatively high compared with that in the upper small intestine, our results may imply that NaHCO3 is an important signaling factor for promoting colonization of EHEC in the lower intestinal tract in humans.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. Phone: 81-3-5449-5250. Fax: 81-3-5449-5250. E-mail: sasakawa{at}ims.u-tokyo.ac.jp.

Editor: V. J. DiRita

Infection and Immunity, July 2002, p. 3500-3509, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3500-3509.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Abuaita, B. H., Withey, J. H. (2009). Bicarbonate Induces Vibrio cholerae Virulence Gene Expression by Enhancing ToxT Activity. Infect. Immun. 77: 4111-4120 [Abstract] [Full Text]  
  • Bhatt, S., Edwards, A. N., Nguyen, H. T. T., Merlin, D., Romeo, T., Kalman, D. (2009). The RNA Binding Protein CsrA Is a Pleiotropic Regulator of the Locus of Enterocyte Effacement Pathogenicity Island of Enteropathogenic Escherichia coli. Infect. Immun. 77: 3552-3568 [Abstract] [Full Text]  
  • House, B., Kus, J. V., Prayitno, N., Mair, R., Que, L., Chingcuanco, F., Gannon, V., Cvitkovitch, D. G., Barnett Foster, D. (2009). Acid-stress-induced changes in enterohaemorrhagic Escherichia coli O157 : H7 virulence. Microbiology 155: 2907-2918 [Abstract] [Full Text]  
  • Kailasan Vanaja, S., Bergholz, T. M., Whittam, T. S. (2009). Characterization of the Escherichia coli O157:H7 Sakai GadE Regulon. J. Bacteriol. 191: 1868-1877 [Abstract] [Full Text]  
  • Lee, D. J., Busby, S. J. W., Westblade, L. F., Chait, B. T. (2008). Affinity Isolation and I-DIRT Mass Spectrometric Analysis of the Escherichia coli O157:H7 Sakai RNA Polymerase Complex. J. Bacteriol. 190: 1284-1289 [Abstract] [Full Text]  
  • Abe, H., Miyahara, A., Oshima, T., Tashiro, K., Ogura, Y., Kuhara, S., Ogasawara, N., Hayashi, T., Tobe, T. (2008). Global Regulation by Horizontally Transferred Regulators Establishes the Pathogenicity of Escherichia coli. DNA Res 15: 25-38 [Abstract] [Full Text]  
  • Walters, M., Sperandio, V. (2006). Autoinducer 3 and Epinephrine Signaling in the Kinetics of Locus of Enterocyte Effacement Gene Expression in Enterohemorrhagic Escherichia coli.. Infect. Immun. 74: 5445-5455 [Abstract] [Full Text]  
  • Iyoda, S., Watanabe, H. (2005). ClpXP Protease Controls Expression of the Type III Protein Secretion System through Regulation of RpoS and GrlR Levels in Enterohemorrhagic Escherichia coli. J. Bacteriol. 187: 4086-4094 [Abstract] [Full Text]  
  • Ferrandiz, M.-J., Bishop, K., Williams, P., Withers, H. (2005). HosA, a Member of the SlyA Family, Regulates Motility in Enteropathogenic Escherichia coli. Infect. Immun. 73: 1684-1694 [Abstract] [Full Text]  
  • Li, M., Rosenshine, I., Tung, S. L., Wang, X. H., Friedberg, D., Hew, C. L., Leung, K. Y. (2004). Comparative Proteomic Analysis of Extracellular Proteins of Enterohemorrhagic and Enteropathogenic Escherichia coli Strains and Their ihf and ler Mutants. Appl. Environ. Microbiol. 70: 5274-5282 [Abstract] [Full Text]  
  • Asahara, T., Shimizu, K., Nomoto, K., Hamabata, T., Ozawa, A., Takeda, Y. (2004). Probiotic Bifidobacteria Protect Mice from Lethal Infection with Shiga Toxin-Producing Escherichia coli O157:H7. Infect. Immun. 72: 2240-2247 [Abstract] [Full Text]  
  • Tatsuno, I., Nagano, K., Taguchi, K., Rong, L., Mori, H., Sasakawa, C. (2003). Increased Adherence to Caco-2 Cells Caused by Disruption of the yhiE and yhiF Genes in Enterohemorrhagic Escherichia coli O157:H7. Infect. Immun. 71: 2598-2606 [Abstract] [Full Text]  
  • Green, B. T., Calvin, A., O'Grady, S. M., Brown, D. R. (2003). Kinin-Induced Anion-Dependent Secretion in Porcine Ileum: Characterization and Involvement of Opioid- and Cannabinoid-Sensitive Enteric Neural Circuits. J. Pharmacol. Exp. Ther. 305: 733-739 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»