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Infection and Immunity, July 2002, p. 3510-3520, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3510-3520.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Effect of Low- and High-Virulence Yersinia enterocolitica Strains on the Inflammatory Response of Human Umbilical Vein Endothelial Cells

Geertrui Denecker,1,2 Sabine Tötemeyer,1,{dagger} Luís J. Mota,1,{ddagger} Paul Troisfontaines,1,{ddagger} Isabelle Lambermont,1 Carine Youta,1 Isabelle Stainier,1 Martin Ackermann,2 and Guy R. Cornelis1,2*

Christian de Duve Institute of Cellular and Molecular Pathology and Faculté de Médecine, Université Catholique de Louvain, Brussels, Belgium,1 Division of Molecular Microbiology, Biozentrum, Universität Basel, Basel, Switzerland2

Received 21 December 2001/ Returned for modification 2 February 2002/ Accepted 13 March 2002

Pathogenic strains of Yersinia spp. inject a set of Yop effector proteins into eukaryotic cells by using a plasmid-encoded type III secretion system. In this study, we analyzed the inflammatory response of human umbilical vein endothelial cells (HUVECs) after infection with different Yersinia enterocolitica strains. We found that both expression of intercellular adhesion molecule 1 and release of the cytokines interleukin-6 (IL-6) and IL-8 by HUVECs are downregulated in a YopP-dependent way, demonstrating that YopP plays a major role in the inflammatory response of these cells. Infection of HUVECs with several low-virulence (biotype 2, 3, and 4) and high-virulence (biotype 1B) Y. enterocolitica strains showed that biotype 1B isolates are more efficient in inhibiting the inflammatory response than low-virulence Y. enterocolitica strains and that this effect depends on the time of contact. We extended the results of Ruckdeschel et al. and found that on the basis of the presence or absence of arginine-143 of YopP (K. Ruckdeschel, K. Richter, O. Mannel, and J. Heesemann, Infect. Immun. 69:7652-7662, 2001) all the Y. enterocolitica strains used fell into two groups, which correlate with the low- and high-virulence phenotypes. In addition, we found that high-virulence strains inject more YopP into the cytosol of eukaryotic target cells than do low-virulence strains.

* Corresponding author. Mailing address: Division of Molecular Microbiology, Biozentrum, Universität Basel, Klingelbergstrasse 50-70, CH-4056 Basel, Switzerland. Phone: 41 61 267 3111. Fax: 41 61 2672118. E-mail: guy.cornelis{at}unibas.ch.

Editor: W. A. Petri, Jr.

{dagger} Present address: Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, United Kingdom.

{ddagger} Present address: Division of Molecular Microbiology, Biozentrum, Universität Basel, CH-4056 Basel, Switzerland.

Infection and Immunity, July 2002, p. 3510-3520, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3510-3520.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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