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Infection and Immunity, July 2002, p. 3586-3591, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3586-3591.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Synergic Effects of Mycoplasmal Lipopeptides and Extracellular ATP on Activation of Macrophages

Takeshi Into,1 Mari Fujita,2 Tsugumi Okusawa,1 Akira Hasebe,1 Manabu Morita,2 and Ken-Ichiro Shibata1*

Departments of Oral Pathobiological Science,1 Oral Health Science, Hokkaido University Graduate School of Dental Medicine, Kita-ku, Sapporo 060-8586, Japan2

Received 2 January 2002/ Returned for modification 14 February 2002/ Accepted 22 March 2002

Mycoplasmal lipopeptides S-(2,3-bispalmitoyloxypropyl)-CGDPKHSPKSF and S-(2,3-bispalmitoyloxypropyl)-CGNNDESNISFKEK activated a monocytic cell line, THP-1 cells, to produce tumor necrosis factor alpha. The activity of the lipopeptides was augmented by ATP in a dose-dependent manner. In addition, the level of expression of mRNAs for tumor necrosis factor alpha and interleukin-1ß, -6, and -8 was also upregulated by the lipopeptides and/or extracellular ATP, but that of interleukin-10 was not. The P2X purinergic receptor antagonists pyridoxal phosphate 6-azophenyl 2',4'-disulfonic acid and periodate-oxidized ATP suppressed the activity of ATP to augment the activation of THP-1 cells by the lipopeptides, suggesting that P2X receptors play important roles in the activity of ATP. The nuclear factor {kappa}B inhibitor dexamethasone also suppressed the activity, suggesting that the activity of ATP is dependent upon the nuclear factor {kappa}B. Thus, these results suggest that the interaction of extracellular ATP with the P2X receptors is attributed to the activity of ATP to augment the activation of THP-1 cells by mycoplasmal lipopeptides.

* Corresponding author. Mailing address: Department of Oral Pathobiological Science, Hokkaido University Graduate School of Dental Medicine, Nishi 7, Kita 13, Kita-ku, Sapporo 060-8586, Japan. Phone: 81(11)706-4240. Fax: 81(11)706-4901. E-mail: shibaken{at}den.hokudai.ac.jp.

Editor: J. D. Clements

Infection and Immunity, July 2002, p. 3586-3591, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3586-3591.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Into, T., Dohkan, J.-i., Inomata, M., Nakashima, M., Shibata, K.-i., Matsushita, K. (2007). Synthesis and Characterization of a Dipalmitoylated Lipopeptide Derived from Paralogous Lipoproteins of Mycoplasma pneumoniae. Infect. Immun. 75: 2253-2259 [Abstract] [Full Text]  
  • Okusawa, T., Fujita, M., Nakamura, J.-i., Into, T., Yasuda, M., Yoshimura, A., Hara, Y., Hasebe, A., Golenbock, D. T., Morita, M., Kuroki, Y., Ogawa, T., Shibata, K.-i. (2004). Relationship between Structures and Biological Activities of Mycoplasmal Diacylated Lipopeptides and Their Recognition by Toll-Like Receptors 2 and 6. Infect. Immun. 72: 1657-1665 [Abstract] [Full Text]  


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