This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Deepe, Jr.,, G. S.
Right arrow Articles by Gibbons, R. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Deepe, Jr.,, G. S.
Right arrow Articles by Gibbons, R. S.

 Previous Article  |  Next Article 

Infection and Immunity, July 2002, p. 3759-3767, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3759-3767.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Cellular and Molecular Regulation of Vaccination with Heat Shock Protein 60 from Histoplasma capsulatum

George S. Deepe Jr.,,1,,2* and Reta S. Gibbons2

Veterans Affairs Hospital,2 Division of Infectious Diseases, University of Cincinnati College of Medicine, Cincinnati, Ohio1

Received 12 February 2002/ Returned for modification 22 March 2002/ Accepted 16 April 2002

Vaccination with heat shock protein 60 (Hsp60) from Histoplasma capsulatum induces a protective immune response in mice. We explored the cellular and molecular requirements for the efficacy of recombinant Hsp60 in mice. Depletion of CD4+, but not CD8+, cells during the inductive phase of vaccination abolished protection, as assessed by survival and by the fungal burden in lungs and spleens. In the expressive phase, the elimination of CD4+ or CD8+ cells after immunization did not significantly alter fungal recovery or survival from a lethal challenge. Depletion of both subpopulations after Hsp60 vaccination resulted in a failure to control a lethal infection and a higher fungal burden in lungs and spleens. Cytokine release by spleen cells from mice vaccinated with Hsp60 produced substantially more gamma interferon and interleukin-10 and -12 than that of cells from mice immunized with either H. capsulatum recombinant Hsp70 or bovine serum albumin. The generation of gamma interferon, but not of interleukin-10, was dependent on T cells, in particular CD4+ cells. Treatment of Hsp60-immunized mice with monoclonal antibody to gamma interferon or interleukin-10 or -12 in the inductive phase of vaccination was accompanied by increased recovery of yeast cells from lungs and spleens and 100% mortality. Likewise, the neutralization of gamma interferon or interleukin-12 abolished the protective effect of Hsp60 in the expressive phase. These results delineate the complexity of the regulatory elements necessary for vaccination against this fungus.

* Corresponding author. Mailing address: Division of Infectious Diseases, Dept. of Medicine, 231 Albert Sabin Way, University of Cincinnati College of Medicine, Cincinnati, OH 45267-0560. Phone: (513) 558-4704. Fax: (513) 558-2089. E-mail: george.deepe{at}uc.edu.

Editor: T. R. Kozel

Infection and Immunity, July 2002, p. 3759-3767, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3759-3767.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Guimaraes, A. J., Frases, S., Gomez, F. J., Zancope-Oliveira, R. M., Nosanchuk, J. D. (2009). Monoclonal Antibodies to Heat Shock Protein 60 Alter the Pathogenesis of Histoplasma capsulatum. Infect. Immun. 77: 1357-1367 [Abstract] [Full Text]  
  • de Bastos Ascenco Soares, R., Gomez, F. J., de Almeida Soares, C. M., Deepe, G. S. Jr. (2008). Vaccination with Heat Shock Protein 60 Induces a Protective Immune Response against Experimental Paracoccidioides brasiliensis Pulmonary Infection. Infect. Immun. 76: 4214-4221 [Abstract] [Full Text]  
  • Scheckelhoff, M., Deepe, G. S. Jr. (2005). A Deficiency in Gamma Interferon or Interleukin-10 Modulates T-Cell-Dependent Responses to Heat Shock Protein 60 from Histoplasma capsulatum. Infect. Immun. 73: 2129-2134 [Abstract] [Full Text]  
  • Steen, B. R., Zuyderduyn, S., Toffaletti, D. L., Marra, M., Jones, S. J. M., Perfect, J. R., Kronstad, J. (2003). Cryptococcus neoformans Gene Expression during Experimental Cryptococcal Meningitis. Eukaryot Cell 2: 1336-1349 [Abstract] [Full Text]  
  • Scheckelhoff, M., Deepe, G. S. Jr. (2002). The Protective Immune Response to Heat Shock Protein 60 of Histoplasma capsulatum Is Mediated by a Subset of V{beta}8.1/8.2+ T Cells. J. Immunol. 169: 5818-5826 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»