This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Henri, S. Articles by Handman, E. Search for Related Content PubMed PubMed Citation Articles by Henri, S. Articles by Handman, E.

 Previous Article  |  Next Article 

Infection and Immunity, July 2002, p. 3874-3880, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3874-3880.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Hierarchy of Susceptibility of Dendritic Cell Subsets to Infection by Leishmania major: Inverse Relationship to Interleukin-12 Production

The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia,¹ Institute of Medical Microbiology, Technical University, Munich, Germany²

Received 30 November 2001/ Returned for modification 30 January 2002/ Accepted 2 April 2002

Dendritic cells (DCs) are professional antigen-presenting cells which initiate and regulate T-cell immune responses. Here we show that murine splenic DCs can be ranked on the basis of their ability to phagocytose and harbor the obligately intracellular parasite Leishmania major. CD4⁺ CD8^- DCs are the most permissive host cells for L. major amastigotes, followed by CD4^- CD8^- DCs; CD4^- CD8⁺ cells are the least permissive. However, the least susceptible CD4^- CD8⁺ DC subset was the best interleukin-12 producer in response to infection. Infection did not induce in any DC subset production of the proinflammatory cytokine gamma interferon and nitric oxide associated with the induction of Th1 responses. The number of parasites phagocytosed by DCs was low, no more than 3 organisms per cell, compared to more than 10 organisms per macrophage. In infected DCs, the parasites are located in a parasitophorous vacuole containing both major histocompatibility complex (MHC) class II and lysosome-associated membrane protein 1 molecules, similar to their location in the infected macrophage. The parasite-driven redistribution of MHC class II to this compartment indicates that infected DCs should be able to present parasite antigen.

Editor: J. M. Mansfield

This article has been cited by other articles:

• Hao, X., Kim, T. S., Braciale, T. J. (2008). Differential Response of Respiratory Dendritic Cell Subsets to Influenza Virus Infection. J. Virol. 82: 4908-4919 [Abstract] [Full Text]  
• Schleicher, U., Liese, J., Knippertz, I., Kurzmann, C., Hesse, A., Heit, A., Fischer, J. A.A., Weiss, S., Kalinke, U., Kunz, S., Bogdan, C. (2007). NK cell activation in visceral leishmaniasis requires TLR9, myeloid DCs, and IL-12, but is independent of plasmacytoid DCs. J. Exp. Med. 204: 893-906 [Abstract] [Full Text]  
• Lahoud, M. H., Proietto, A. I., Gartlan, K. H., Kitsoulis, S., Curtis, J., Wettenhall, J., Sofi, M., Daunt, C., O'Keeffe, M., Caminschi, I., Satterley, K., Rizzitelli, A., Schnorrer, P., Hinohara, A., Yamaguchi, Y., Wu, L., Smyth, G., Handman, E., Shortman, K., Wright, M. D. (2006). Signal Regulatory Protein Molecules Are Differentially Expressed by CD8- Dendritic Cells. J. Immunol. 177: 372-382 [Abstract] [Full Text]  
• Prickett, S., Gray, P. M., Colpitts, S. L., Scott, P., Kaye, P. M., Smith, D. F. (2006). In vivo recognition of ovalbumin expressed by transgenic leishmania is determined by its subcellular localization.. J. Immunol. 176: 4826-4833 [Abstract] [Full Text]  
• Korner, U., Fuss, V., Steigerwald, J., Moll, H. (2006). Biogenesis of Leishmania major-Harboring Vacuoles in Murine Dendritic Cells. Infect. Immun. 74: 1305-1312 [Abstract] [Full Text]  
• Breton, M., Tremblay, M. J, Ouellette, M., Papadopoulou, B. (2005). Live Nonpathogenic Parasitic Vector as a Candidate Vaccine against Visceral Leishmaniasis. Infect. Immun. 73: 6372-6382 [Abstract] [Full Text]  
• Baldwin, T., Henri, S., Curtis, J., O'Keeffe, M., Vremec, D., Shortman, K., Handman, E. (2004). Dendritic Cell Populations in Leishmania major-Infected Skin and Draining Lymph Nodes. Infect. Immun. 72: 1991-2001 [Abstract] [Full Text]  
• Lemos, M. P., Esquivel, F., Scott, P., Laufer, T. M. (2004). MHC Class II Expression Restricted to CD8{alpha}+ and CD11b+ Dendritic Cells Is Sufficient for Control of Leishmania major. J. Exp. Med. 199: 725-730 [Abstract] [Full Text]  
• Prina, E., Abdi, S. Z., Lebastard, M., Perret, E., Winter, N., Antoine, J.-C. (2004). Dendritic cells as host cells for the promastigote and amastigote stages of Leishmania amazonensis: the role of opsonins in parasite uptake and dendritic cell maturation. J. Cell Sci. 117: 315-325 [Abstract] [Full Text]  
• Hogg, K. G., Kumkate, S., Anderson, S., Mountford, A. P. (2003). Interleukin-12 p40 Secretion by Cutaneous CD11c+ and F4/80+ Cells Is a Major Feature of the Innate Immune Response in Mice That Develop Th1-Mediated Protective Immunity to Schistosomamansoni. Infect. Immun. 71: 3563-3571 [Abstract] [Full Text]  
• Muraille, E., De Trez, C., Pajak, B., Torrentera, F. A., De Baetselier, P., Leo, O., Carlier, Y. (2003). Amastigote Load and Cell Surface Phenotype of Infected Cells from Lesions and Lymph Nodes of Susceptible and Resistant Mice Infected with Leishmania major. Infect. Immun. 71: 2704-2715 [Abstract] [Full Text]