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Infection and Immunity, July 2002, p. 3930-3934, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3930-3934.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Magnesium Uptake by CorA Is Essential for Viability of the Gastric Pathogen Helicobacter pylori

Jens Pfeiffer, Johannes Guhl, Barbara Waidner, Manfred Kist, and Stefan Bereswill*

Department of Medical Microbiology and Hygiene, Institute of Medical Microbiology and Hygiene, University Hospital of Freiburg, D-79104 Freiburg, Germany

Received 6 February 2002/ Returned for modification 18 March 2002/ Accepted 2 April 2002

We show here that Mg2+ acquisition by CorA is essential for Helicobacter pylori in vitro, as corA mutants did not grow in media without Mg2+ supplementation. Complementation analysis performed with an Escherichia coli corA mutant revealed that H. pylori CorA transports nickel and cobalt in addition to Mg2+. However, Mg2+ is the dominant CorA substrate, as the corA mutation affected neither cobalt and nickel resistance nor nickel induction of urease in H. pylori. The drastic Mg2+ requirement (20 mM) of H. pylori corA mutants indicates that CorA plays a key role in the adaptation to the low-Mg2+ conditions predominant in the gastric environment.

* Corresponding author. Mailing address: Department of Medical Microbiology and Hygiene, Institute of Medical Microbiology and Hygiene, University Hospital of Freiburg, Hermann-Herder-Str. 11, D-79104 Freiburg, Germany. Phone: 49-761-203-6539. Fax: 49-761-203-6562. E-mail: bereswil{at}ukl.uni-freiburg.de.

Editor: J. T. Barbieri

Infection and Immunity, July 2002, p. 3930-3934, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3930-3934.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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