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Infection and Immunity, July 2002, p. 3944-3947, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3944-3947.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Cysteine Protease Activity and Histamine Release from the Human Mast Cell Line HMC-1 Stimulated by Recombinant Streptococcal Pyrogenic Exotoxin B/Streptococcal Cysteine Protease

Yukino Watanabe,1 Yuko Todome,1 Hisashi Ohkuni,1,2* Shinsaku Sakurada,3 Toshio Ishikawa,1 Takashi Yutsudo,4 Vincent A. Fischetti,5 and John B. Zabriskie6

Department of Immunology and Infectious Diseases, Institute of Gerontology, Nippon Medical School, Nakahara-ku, Kawasaki 211-8533,1 Clinical Laboratory, Medca Japan Co., Ltd., Tenjin, Konosu, Saitama 365-8511,2 Bureau of International Cooperation, International Medical Center of Japan Tokyo 162-8655,3 Medical Science, Discovery Research Laboratory, Shionogi & Co., Ltd., Mishima Settsu-shi, Osaka 566-0022, Japan,4 Laboratory of Bacterial Pathogenesis and Immunology,5 Laboratory of Clinical Microbiology and Immunology, Rockefeller University, New York, New York 10021-63996

Received 7 February 2002/ Returned for modification 7 March 2002/ Accepted 27 March 2002

We constructed the expression vector pSK-SCP containing the streptococcal exotoxin B gene (spe b) which expressed protease activity. We showed that the recombinant streptococcal pyogenic exotoxin B/streptococcal cysteine protease (rSPE B/SCP) was secreted into the culture supernatant of the transformant and retained its SCP activity, which was equivalent to or greater than that of the naturally occurring molecule. The secreted rSPE B/SCP induced histamine release and degranulation of the human mast cell line HMC-1. This study may contribute to the understanding of the pathogenic role of SPE B/SCP in streptococcal infection and streptococcal toxic shock syndrome.

* Corresponding author. Mailing address: Clinical Laboratory, Medca Japan Co., Ltd., 3-673 Tenjin, Konosu-shi, Saitama 365-8511, Japan. Phone: 81-48-540-6581. Fax: 81-48-540-6581. E-mail: h-okuni{at}nms.ac.jp.

Editor: J. T. Barbieri

Infection and Immunity, July 2002, p. 3944-3947, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3944-3947.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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