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Infection and Immunity, July 2002, p. 3948-3952, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3948-3952.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Sigma B Contributes to PrfA-Mediated Virulence in Listeria monocytogenes

Celine A. Nadon, Barbara M. Bowen, Martin Wiedmann, and Kathryn J. Boor*

Department of Food Science, Cornell University, Ithaca, New York

Received 20 February 2002/ Returned for modification 18 March 2002/ Accepted 4 April 2002

Transcription of the Listeria monocytogenes positive regulatory factor A protein (PrfA) is initiated from either of two promoters immediately upstream of prfA (prfAp1 and prfAp2) or from the upstream plcA promoter. We demonstrate that prfAp2 is a functional {sigma}B-dependent promoter and that a sigB deletion mutation affects the virulence phenotype of L. monocytogenes. Thus, the alternative sigma factor {sigma}B contributes to virulence in L. monocytogenes.

* Corresponding author. Mailing address: Department of Food Science, 413 Stocking Hall, Cornell University, Ithaca, NY 14853. Phone: (607) 255-3111. Fax: (607) 254-4868. E-mail: kjb4{at}cornell.edu.

Editor: J. T. Barbieri

Infection and Immunity, July 2002, p. 3948-3952, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3948-3952.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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