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Infection and Immunity, July 2002, p. 3973-3977, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3973-3977.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Activation of the Pseudomonas aeruginosa Type III Secretion System Requires an Intact Pyruvate Dehydrogenase aceAB Operon

Denis Dacheux,1,2 Olivier Epaulard,1,3 Arjan de Groot,1 Benoit Guery,4 Rozen Leberre,4 Ina Attree,1 Benoit Polack,3 and Bertrand Toussaint1,5*

Département de Biologie Moléculaire et Structurale, BBSI, UMR 5092 CNRS, CEA-Grenoble,1 GREPI EA 2938 MENRT,3 DBPC/Enzymologie,,5 CHU-Grenoble, Grenoble, Université de Bordeaux, Paris, and,2 Réanimation Médicale et Maladies Infectieuses, CH Dron, Tourcoing, France4

Received 23 January 2002/ Returned for modification 21 March 2002/ Accepted 15 April 2002

Pseudomonas aeruginosa clinical cystic fibrosis isolate CHA was mutagenized with Tn5Tc to identify new genes involved in type III secretion system (TTSS)-dependent cytotoxicity toward human polymorphonuclear neutrophils. Among 25 mutants affected in TTSS function, 14 contained the insertion at different positions in the aceAB operon encoding the PDH-E1 and -E2 subunits of pyruvate dehydrogenase. In PDH mutants, no transcriptional activation of TTSS genes in response to calcium depletion occurred. Expression in trans of ExsA restored TTSS function and cytotoxicity.

* Corresponding author. Mailing address: DBPC/Enzymologie, CHU-Grenoble BP217, 38043 Grenoble cedex, France. Phone: 33.4.76.76.54.83. Fax: 33.4.76.76.56.08. E-mail: btoussaint{at}chu-grenoble.fr.

Editor: E. I. Tuomanen

Infection and Immunity, July 2002, p. 3973-3977, Vol. 70, No. 7
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.7.3973-3977.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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