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Infection and Immunity, August 2002, p. 4254-4260, Vol. 70, No. 8
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.8.4254-4260.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Induction of Cell-Mediated Immunity to Staphylococcus aureus in the Mouse Mammary Gland by Local Immunization with a Live Attenuated Mutant

Marisa I. Gómez, Daniel O. Sordelli,* Fernanda R. Buzzola, and Verónica E. García

Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, 1121 Buenos Aires, Argentina

Received 10 December 2001/ Returned for modification 6 March 2002/ Accepted 30 April 2002

The efficacy of intramammary (Ima) immunization with a live attenuated (la) Staphylococcus aureus mutant to protect the mouse mammary gland from infection has previously been established. The present study was aimed at evaluating whether Ima immunization with la-S. aureus can induce cell-mediated immune responses to the pathogen within the mammary gland. Mice were immunized by Ima route with la-S. aureus, and regional lymph node mononuclear cells were obtained thereafter. A higher expression of the interleukin-2 receptor was found on B and T cells from immunized mice when they were compared with control mice. Immunization with la-S. aureus induced strong proliferative responses to S. aureus. Moreover, significantly increased levels of gamma interferon (IFN-{gamma}) were produced by CD4+ T cells when lymphocytes from immunized mice, but not from control mice, were cultured in the presence of staphylococcal antigens. Moreover, a significant increase in the percentage of IFN-{gamma}-producing CD4+ and CD8+ T cells was observed after S. aureus Ima challenge in immunized mice compared to challenged control mice. Our results demonstrated that Ima immunization with la-S. aureus induced primed lymphocyte populations capable of responding against staphylococcal antigens during in vitro stimulation, as well as during in vivo infection by S. aureus. CD4+ and CD8+ T cells appear to be the main lymphocyte subpopulations involved in this response. It is suggested that IFN-{gamma} production induced by Ima immunization may play a pivotal role in the eradication of intracellular staphylococci.

* Corresponding author. Mailing address: Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155 P-12, 1121 Buenos Aires, Argentina. Phone: 54-11-4963-6669. Fax: 54-11-4508-3705. E-mail: sordelli{at}fmed.uba.ar.

Editor: V. J. DiRita

Infection and Immunity, August 2002, p. 4254-4260, Vol. 70, No. 8
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.8.4254-4260.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Buzzola, F. R., Barbagelata, M. S., Caccuri, R. L., Sordelli, D. O. (2006). Attenuation and Persistence of and Ability To Induce Protective Immunity to a Staphylococcus aureus aroA Mutant in Mice.. Infect. Immun. 74: 3498-3506 [Abstract] [Full Text]  


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