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Contribution of Phospholipomannan to the Surface Expression of ß-1,2-Oligomannosides in Candida albicans and Its Presence in Cell Wall Extracts

Laboratoire de Mycologie Fondamentale et Appliquée, INSERM EPI 9915, Faculté de Médecine, Pôle Recherche, 59045 Lille Cedex,¹ Laboratoire de Physiologie et Physiopathologie Hormonale de la Prostate Humaine, INSERM EPI 9938, Université des Sciences et Techniques, 59655 Villeneuve d'Ascq Cedex, France,² Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanità, 00161 Rome, Italy³

Received 26 October 2001/ Returned for modification 2 December 2001/ Accepted 16 April 2002

ß-1,2-Oligomannosides (ß-1,2-Man) derived from Candida albicans mannan have been shown to act as adhesins and to induce protective antibodies. We used monoclonal antibodies specific for ß-1,2-Man in electron, confocal, and fluorescence microscopy to study the surface expression of ß-1,2-Man epitopes. These monoclonal antibodies were also used for Western blotting of cell surface extracts to study the nature of the molecules expressing the ß-Man epitopes. Evidence was obtained for the contribution of a glycolipid, phospholipomannan (PLM), to the complex expression of ß-1,2-Man epitopes at the cell wall surfaces of yeasts grown on solid media. PLM was present in intercellular matrixes of colonies grown on agar and was detected as a contaminant in mannan batches prepared by conventional methods.

Editor: T. R. Kozel

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