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Infection and Immunity, August 2002, p. 4323-4328, Vol. 70, No. 8
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.8.4323-4328.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Contribution of Phospholipomannan to the Surface Expression of ß-1,2-Oligomannosides in Candida albicans and Its Presence in Cell Wall Extracts

D. Poulain,1* C. Slomianny,2 T. Jouault,1 J. M. Gomez,3 and P. A. Trinel1

Laboratoire de Mycologie Fondamentale et Appliquée, INSERM EPI 9915, Faculté de Médecine, Pôle Recherche, 59045 Lille Cedex,1 Laboratoire de Physiologie et Physiopathologie Hormonale de la Prostate Humaine, INSERM EPI 9938, Université des Sciences et Techniques, 59655 Villeneuve d'Ascq Cedex, France,2 Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanità, 00161 Rome, Italy3

Received 26 October 2001/ Returned for modification 2 December 2001/ Accepted 16 April 2002

ß-1,2-Oligomannosides (ß-1,2-Man) derived from Candida albicans mannan have been shown to act as adhesins and to induce protective antibodies. We used monoclonal antibodies specific for ß-1,2-Man in electron, confocal, and fluorescence microscopy to study the surface expression of ß-1,2-Man epitopes. These monoclonal antibodies were also used for Western blotting of cell surface extracts to study the nature of the molecules expressing the ß-Man epitopes. Evidence was obtained for the contribution of a glycolipid, phospholipomannan (PLM), to the complex expression of ß-1,2-Man epitopes at the cell wall surfaces of yeasts grown on solid media. PLM was present in intercellular matrixes of colonies grown on agar and was detected as a contaminant in mannan batches prepared by conventional methods.

* Corresponding author. Mailing address: Laboratoire de Mycologie Fondamentale et Appliquée, INSERM EPI 9915, Faculté de Médecine, Pôle Recherche, Place de Verdun, 59045 Lille Cedex, France. Phone: 33-3 20 62 34 20. Fax: 33-3 20 62 34 16. E-mail: dan_poulain{at}compuserve.com.

Editor: T. R. Kozel

Infection and Immunity, August 2002, p. 4323-4328, Vol. 70, No. 8
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.8.4323-4328.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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