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Infection and Immunity, August 2002, p. 4344-4352, Vol. 70, No. 8
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.8.4344-4352.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Differential Expression of Virulence-Related Genes in Enterococcus faecalis in Response to Biological Cues in Serum and Urine

Brett D. Shepard1 and Michael S. Gilmore1,2*

Department of Ophthalmology, Dean A. McGee Eye Institute,2 Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 731041

Received 14 January 2002/ Returned for modification 7 March 2002/ Accepted 19 April 2002

Enterococci rank among leading causes of nosocomial bacteremia and urinary tract infection and are also a leading cause of community acquired subacute endocarditis. Limited evidence suggests that biological cues in serum and urine may play an important role in modulating enterococcal virulence at sites of infection. To determine the extent to which biological cues affect enterococcal virulence-associated gene expression, we used quantitative real-time PCR to compare mRNA levels in Enterococcus faecalis cultures grown in serum or urine to that achieved in laboratory medium. Both environment- and growth phase-specific variations were observed, demonstrating the occurrence of as-yet-uncharacterized mechanisms for control of gene expression in E. faecalis that may play an important role in vivo.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Department of Ophthalmology, 975 N.E. 10th St., BRC 356, Oklahoma City, OK 73104. Phone: (405) 271-7969. Fax: (405) 271-3013. E-mail: michael-gilmore{at}ouhsc.edu.

Editor: E. I Tuomanen

Infection and Immunity, August 2002, p. 4344-4352, Vol. 70, No. 8
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.8.4344-4352.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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