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Infection and Immunity, August 2002, p. 4708-4715, Vol. 70, No. 8
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.8.4708-4715.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Role of Polyphosphate Kinase in Biofilm Formation by Porphyromonas gingivalis

Wen Chen,1 Robert J. Palmer,2 and Howard K. Kuramitsu1*

Department of Oral Biology, State University of New York, Buffalo, New York 14214,1 Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 208922

Received 4 March 2002/ Returned for modification 10 April 2002/ Accepted 16 May 2002

In order to assess the role of polyphosphate kinase (PPK) in the physiology of Porphyromonas gingivalis, a ppk gene mutant, CW120, was constructed and characterized. P. gingivalis was demonstrated to synthesize short-chain polyphosphate (polyP) but not long-chain polyP. CW120 failed to survive in the stationary phase as well as the parental cell did, and it was attenuated in biofilm formation on polyvinylchloride and glass surfaces. Furthermore, the complementation by insertion of an intact copy of the ppk gene into the mutant CW120 restored its biofilm formation and stationary-phase survival. These results suggest that PPK may be important for incorporation of these organisms into subgingival plaque in the human oral cavity.

* Corresponding author. Mailing address: Department of Oral Biology, State University of New York at Buffalo, 3435 Main St., Buffalo, NY 14214. Phone: (716) 829-2068. Fax: (716) 829-3942. E-mail: KURAMITS{at}BUFFALO.EDU.

Editor: E. I. Tuomanen

Infection and Immunity, August 2002, p. 4708-4715, Vol. 70, No. 8
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.8.4708-4715.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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