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Infection and Immunity, August 2002, p. 4729-4734, Vol. 70, No. 8
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.8.4729-4734.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification of Klebsiella pneumoniae Genes Involved in Intestinal Colonization and Adhesion Using Signature-Tagged Mutagenesis

Nathalie Maroncle, Damien Balestrino, Chantal Rich, and Christiane Forestier*

Laboratoire de Bactériologie, Université d'Auvergne, Faculté de Pharmacie, 63000 Clermont-Ferrand, France

Received 4 December 2001/ Returned for modification 6 March 2002/ Accepted 6 May 2002

Klebsiella pneumoniae is an opportunistic pathogen responsible for nosocomial infections that initially colonize the intestinal tract of patients. Signature-tagged mutagenesis was used to identify genes required for this function. A library of 2,200 mutants was analyzed for the inability of the mutants to survive in a murine model of intestinal colonization and to adhere to human intestinal cells (Int-407) in vitro. Twenty-nine attenuated mutants were selected for further analyses after competition assays against the wild-type strain. Whatever the screening model, most of the transposon insertions occurred in genes involved in metabolic pathways, membrane transport, DNA metabolism, transcriptional regulation, and unknown functions. Only one mutant was attenuated in both the murine colonization and the in vitro adhesion models, and the sequence disrupted by the transposon had homology to adhesin-encoding genes of Haemophilus sp.

* Corresponding author. Mailing address: Laboratoire de Bactériologie, Université d'Auvergne, Faculté de Pharmacie, 28 Place H. Dunant, 63000 Clermont-Ferrand, France. Phone: (33) 4 73 17 79 94. Fax: (33) 4 73 27 74 94. E-mail: Christiane.forestier{at}u-clermont1.fr.

Editor: V. J. DiRita

Infection and Immunity, August 2002, p. 4729-4734, Vol. 70, No. 8
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.8.4729-4734.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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