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Infection and Immunity, August 2002, p. 4747-4749, Vol. 70, No. 8
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.8.4747-4749.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Phase Variation Analysis of Coxiella burnetii during Serial Passage in Cell Culture by Use of Monoclonal Antibodies

Akitoyo Hotta, Midori Kawamura, Ho To, Masako Andoh, Tsuyoshi Yamaguchi, Hideto Fukushi, and Katsuya Hirai*

Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Gifu 501-1193, Japan

Received 19 December 2001/ Returned for modification 4 March 2002/ Accepted 9 May 2002

Antigenic changes in Coxiella burnetii Nine Mile strain phase I during serial passages in cell culture were analyzed with three groups of monoclonal antibodies (MAbs) against lipopolysaccharide. The MAbs of group 1 did not react with organisms that were passaged over five times, and the MAbs of group 2 did not react with organisms that were passaged over eight times. The MAbs of group 3 reacted with organisms passaged up to 15 times but did not react with phase II cells. These results suggest that C. burnetii could be differentiated into four phase states during phase variation.

* Corresponding author. Mailing address: Katsuya Hirai Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan. Phone and fax: 81-58-293-2945. E-mail: khirai{at}cc.gifu-u.ac.jp.

Editor: R. N. Moore

Infection and Immunity, August 2002, p. 4747-4749, Vol. 70, No. 8
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.8.4747-4749.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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  • Andoh, M., Naganawa, T., Hotta, A., Yamaguchi, T., Fukushi, H., Masegi, T., Hirai, K. (2003). SCID Mouse Model for Lethal Q Fever. Infect. Immun. 71: 4717-4723 [Abstract] [Full Text]  
  • Hotta, A., Kawamura, M., To, H., Andoh, M., Yamaguchi, T., Fukushi, H., Amano, K.-I., Hirai, K. (2003). Use of Monoclonal Antibodies to Lipopolysaccharide for Antigenic Analysis of Coxiella burnetii. J. Clin. Microbiol. 41: 1747-1749 [Abstract] [Full Text]  


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