This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Oho, T.
Right arrow Articles by Koga, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oho, T.
Right arrow Articles by Koga, T.

 Previous Article  |  Next Article 

Infection and Immunity, September 2002, p. 5279-5282, Vol. 70, No. 9
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.9.5279-5282.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Functional Domain of Bovine Milk Lactoferrin Which Inhibits the Adherence of Streptococcus mutans Cells to a Salivary Film{dagger}

Takahiko Oho,* Morihide Mitoma, and Toshihiko Koga,{ddagger}

Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812-8582, Japan

Received 28 January 2002/ Returned for modification 9 April 2002/ Accepted 6 June 2002

The bovine lactoferrin molecule and relatively long lactoferrin fragments containing residues 473 to 538 strongly inhibited adherence of Streptococcus mutans to saliva-coated hydroxyapatite beads. Each cysteine residue in Lf411 (residues 473 to 538) was replaced by a serine residue, and the mutants Lf411-C481S and Lf411-C532S strongly inhibited S. mutans adherence. These results suggest that the functional domain of lactoferrin that binds to a salivary film lies in residues 473 to 538 and that the region might be concealed by disulfide bond formation between Cys481 and Cys532 in the Lf411 fragment.

* Corresponding author. Mailing address: Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Phone: 81-92-642-6353. Fax: 81-92-642-6354. E-mail: oho{at}dent.kyushu-u.ac.jp.

{dagger} This paper is dedicated to the memory of Toshihiko Koga.

Editor: V. J. DiRita

{ddagger} Deceased.

Infection and Immunity, September 2002, p. 5279-5282, Vol. 70, No. 9
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.9.5279-5282.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Oho, T., Bikker, F. J., Nieuw Amerongen, A. V., Groenink, J. (2004). A Peptide Domain of Bovine Milk Lactoferrin Inhibits the Interaction between Streptococcal Surface Protein Antigen and a Salivary Agglutinin Peptide Domain. Infect. Immun. 72: 6181-6184 [Abstract] [Full Text]  
  • Di Biase, A. M., Tinari, A., Pietrantoni, A., Antonini, G., Valenti, P., Conte, M. P., Superti, F. (2004). Effect of bovine lactoferricin on enteropathogenic Yersinia adhesion and invasion in HEp-2 cells. J Med Microbiol 53: 407-412 [Abstract] [Full Text]  
  • Aimutis, W. R. (2004). Bioactive Properties of Milk Proteins with Particular Focus on Anticariogenesis. J. Nutr. 134: 989S-995S [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»