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Infection and Immunity, September 2002, p. 5287-5289, Vol. 70, No. 9
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.9.5287-5289.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Susceptibility of Nontypeable Haemophilus influenzae to Human ß-Defensins Is Influenced by Lipooligosaccharide Acylation

Timothy D. Starner,¹ W. Edward Swords,^2, Michael A. Apicella,² and Paul B. McCray, Jr.^1*

Department of Pediatrics,¹ Department of MicrobiologyThe University of Iowa, Iowa City, Iowa 52242²

Received 28 February 2002/ Returned for modification 29 April 2002/ Accepted 16 May 2002

Nontypeable Haemophilus influenzae (NTHI) lipooligosaccharide htrB mutants exhibited greater than 45-fold-increased sensitivity to human ß-defensin 2 (HBD-2) compared to the wild type. Complementation by htrB in trans to acylation competence reversed this increased sensitivity. In contrast, NTHI was more susceptible to HBD-3 and showed no changes in sensitivity as a result of lipooligosaccharide mutations in oligosaccharide and lipid A biosynthesis genes.

Editor: E. I. Tuomanen

Present address: Department of Microbiology & Immunology, Wake Forest University School of Medicine, Winston-Salem, NC 27157.

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