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Infection and Immunity, September 2002, p. 5295-5298, Vol. 70, No. 9
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.9.5295-5298.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Differential Expression of the p44 Gene Family in the Agent of Human Granulocytic Ehrlichiosis

Jacob W. IJdo,1* Caiyun Wu,2 Sam R. Telford III,3 and Erol Fikrig2

Inflammation Program and Departments of Internal Medicine, Veterans Administration Medical Center and University of Iowa, Iowa City, Iowa,1 Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut,2 Laboratory of Public Health Entomology, Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts3

Received 5 March 2002/ Returned for modification 3 April 2002/ Accepted 25 May 2002

Using reverse transcription-PCR targeting of the p44 genes of the agent of human granulocytic ehrlichiosis (HGE) with primers flanking the hypervariable region, we show differential expression in a murine model of HGE infection and during tick transmission. The p44 genes were differentially expressed in salivary glands of infected nymphal ticks removed during transmission feeding but not in nonfeeding infected ticks. Similarly, the p44 genes were differentially expressed in infected C3H mice, in SCID mice, and in cultured HGE bacteria. Thus, differential p44 expression exists in vivo and in vitro and could provide a basis for antigenic variation.

* Corresponding author. Mailing address: Division of Rheumatology, C312GH, University of Iowa College of Medicine, 200 Hawkins Dr., Iowa City, IA 52242. Phone: (319) 384-6175. Fax: (319) 353-6290. E-mail: jacob-ijdo{at}uiowa.edu.

Editor: W. A. Petri, Jr.

Infection and Immunity, September 2002, p. 5295-5298, Vol. 70, No. 9
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.9.5295-5298.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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