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Infection and Immunity, January 2003, p. 109-116, Vol. 71, No. 1
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.1.109-116.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Generation of Helicobacter pylori Ghosts by PhiX Protein E-Mediated Inactivation and Their Evaluation as Vaccine Candidates

Klaus Panthel,1 Wolfgang Jechlinger,2 Alexander Matis,2 Manfred Rohde,3 Michael Szostak,4 Werner Lubitz,2,4 and Rainer Haas1*

Max von Pettenkofer Institute for Hygiene and Medical Microbiology, LMU Munich,1 Department of Microbial Pathogenicity and Vaccine Research, GBF Braunschweig, Germany,3 Institute of Microbiology and Genetics, University of Vienna,2 BIRD-C GmbH, Vienna, Austria4

Received 22 July 2002/ Returned for modification 24 September 2002/ Accepted 15 October 2002

Bacterial ghosts are empty cell envelopes, which may be generated by the controlled expression of the PhiX174 lysis gene E in gram-negative bacteria to obtain vaccine candidates. We describe here the application of this technology to Helicobacter pylori. The lysis gene cassette was cloned into an Escherichia coli-Helicobacter pylori shuttle vector and introduced into an H. pylori recipient strain by bacterial conjugation. Temperature induction of the lysis gene cassette revealed a quantitative killing of the H. pylori culture without induction of lysis-resistant bacteria. Biochemical and transmission electron microscopic studies identified structurally intact H. pylori. Prophylactic oral vaccination experiments using these H. pylori ghosts in the BALB/c mouse model showed a significant reduction of the bacterial load in the ghost group, as measured by a quantitative bacterial reisolation procedure. Ten of 10 and 5 of 10 mice were protected, respectively, without the use of a mucosal adjuvant. Coadministration of ghosts with cholera toxin as mucosal adjuvant resulted in a complete protection of 10 of 10 and 8 of 8 mice against H. pylori challenge, with three animals showing a sterile immunity.

* Corresponding author. Mailing address: Max von Pettenkofer-Institute for Hygiene and Medical Microbiology, Pettenkoferstr. 9a, D-80336 Munich, Germany. Phone: (49)-89-5160 5255. Fax: (49)-89-5160 5223. E-mail: haas{at}m3401.mpk.med.uni-muenchen.de.

Editor: D. L. Burns

Infection and Immunity, January 2003, p. 109-116, Vol. 71, No. 1
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.1.109-116.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bumann, D., Habibi, H., Kan, B., Schmid, M., Goosmann, C., Brinkmann, V., Meyer, T. F., Jungblut, P. R. (2004). Lack of Stage-Specific Proteins in Coccoid Helicobacter pylori Cells. Infect. Immun. 72: 6738-6742 [Abstract] [Full Text]  
  • Panthel, K., Dietz, P., Haas, R., Beier, D. (2003). Two-Component Systems of Helicobacter pylori Contribute to Virulence in a Mouse Infection Model. Infect. Immun. 71: 5381-5385 [Abstract] [Full Text]  


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