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Infection and Immunity, January 2003, p. 234-241, Vol. 71, No. 1
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.1.234-241.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Seeligeriolysin O, a Cholesterol-Dependent Cytolysin of Listeria seeligeri, Induces Gamma Interferon from Spleen Cells of Mice

Yutaka Ito, Ikuo Kawamura, Chikara Kohda, Hisashi Baba,{dagger} Takamasa Nomura, Terumi Kimoto, Isao Watanabe, and Masao Mitsuyama*

Department of Microbiology, Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan

Received 7 June 2002/ Returned for modification 17 July 2002/ Accepted 12 September 2002

Seeligeriolysin O (LSO), one of the cholesterol-dependent cytolysins produced by Listeria seeligeri, shows 80% homology to listeriolysin O (LLO) produced by Listeria monocytogenes at the amino acid sequence level. In addition to cytolytic activity, LLO has been shown to exhibit cytokine-inducing activity. In order to determine whether LSO is also capable of exhibiting these two different activities, we constructed a recombinant full-length LSO (rLSO530) and a noncytolytic truncated derivative with a C-terminal deletion (rLSO483) and compared these molecules with recombinant LLO. The cytolytic rLSO530 molecule could induce gamma interferon (IFN-{gamma}) production in spleen cells when the cytolytic activity was blocked by treatment with cholesterol. The noncytolytic truncated rLSO483 molecule also induced IFN-{gamma} production. Anti-LLO polyclonal antibody inhibited not only LLO-induced IFN-{gamma} production but also LSO-induced IFN-{gamma} production. Both NK cells and CD11b+ cells were required for LSO-induced IFN-{gamma} production. Among the various cytokines expressed in CD11b+ cells, interleukin-12 (IL-12) and IL-18 appeared to be essential. We concluded that LSO exhibits the same biological activity as LLO.

* Corresponding author. Mailing address: Department of Microbiology, Kyoto University Graduate School of Medicine, Yoshida Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan. Phone: 81-75-753-4441. Fax: 81-75-753-4446. E-mail: mituyama{at}mb.med.kyoto-u.ac.jp.

Editor: J. D. Clements

{dagger} Present address: First Department of Internal Medicine, Nagoya University School of Medicine, Nagoya 466-8550, Japan.

Infection and Immunity, January 2003, p. 234-241, Vol. 71, No. 1
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.1.234-241.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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