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Infection and Immunity, January 2003, p. 317-326, Vol. 71, No. 1
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.1.317-326.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Expression of L-Selectin (CD62L), CD44, and CD25 on Activated Bovine T Cells

W. R. Waters,1* T. E. Rahner,1 M. V. Palmer,1 D. Cheng,2 B. J. Nonnecke,3 and D. L. Whipple1

Bacterial Diseases of Livestock Research Unit,1 Periparturient Diseases of Cattle Research Unit, National Animal Disease Center, USDA Agricultural Research Service, Ames, Iowa 50010,3 Flow Cytometry Facility, Iowa State University, Ames, Iowa 500112

Received 20 June 2002/ Returned for modification 7 August 2002/ Accepted 15 October 2002

Mycobacterium bovis infection of cattle represents a natural host-pathogen interaction and, in addition to its economic and zoonotic impact, represents a model for human tuberculosis. Extravasation and trafficking of activated lymphocytes to inflammatory sites is modulated by differential expression of multiple surface adhesion molecules. However, effects of M. bovis infection on adhesion molecule expression have not been characterized. To determine these changes, peripheral blood mononuclear cells from M. bovis-infected cattle were stimulated with M. bovis purified protein derivative (PPD) or pokeweed mitogen (PWM) and evaluated concurrently for proliferation and activation marker expression. Stimulation with PPD or PWM increased CD25 and CD44 mean fluorescence intensity (MFI) and decreased CD62L MFI on CD4+ cells from infected animals. CD62L MFI on PPD- and PWM-stimulated {gamma}{delta} T-cell receptor-positive (TCR+) and CD8+ cells was also reduced compared to that of nonstimulated {gamma}{delta} TCR+ and CD8+ cells. Using a flow cytometry-based proliferation assay, it was determined that proliferating cells, regardless of lymphocyte subset, exhibited increased expression of CD25 and CD44 and decreased expression of CD62L compared to cells that had not proliferated. In contrast to proliferation, activation-induced apoptosis of CD4+ cells resulted in a significant down regulation of CD44 expression. Lymphocytes obtained from lungs of M. bovis-infected cattle also had reduced expression of CD44 compared to lymphocytes from lungs of noninfected cattle. These alterations in surface molecule expression upon activation likely impact trafficking to sites of inflammation and the functional capacity of these cells within tuberculous granulomas.

* Corresponding author. Mailing address: United States Department of Agriculture, Agricultural Research Service, National Animal Disease Center, P.O. Box 70, Ames, IA 50010-0070. Phone: (515) 663-7756. Fax: (515) 663-7458. E-mail: rwaters{at}nadc.ars.usda.gov.

Editor: B. B. Finlay

Infection and Immunity, January 2003, p. 317-326, Vol. 71, No. 1
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.1.317-326.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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