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Infection and Immunity, January 2003, p. 418-427, Vol. 71, No. 1
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.1.418-427.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The Salmonella enterica Serovar Typhimurium Translocated Effectors SseJ and SifB Are Targeted to the Salmonella-Containing Vacuole

Jeremy A. Freeman,1,{dagger} Michael E. Ohl,2 and Samuel I. Miller1,2,3*

Departments of Microbiology,1 Medicine,2 Genome Sciences, University of Washington, Seattle, Washington 981953

Received 20 May 2002/ Returned for modification 3 July 2002/ Accepted 16 September 2002

The Salmonella enterica serovar Typhimurium type III secretion system (TTSS) encoded in Salmonella pathogenicity island 2 (SPI-2) promotes replication within host cells and systemic infection of mice. The SPI-2 TTSS is expressed following Salmonella internalization into host cells and translocates effectors across the membrane of the Salmonella-containing vacuole (SCV). Two effectors with similar amino-terminal domains, SseJ and SifB, localize to the SCV membrane in infected HEp-2 cells and subsequently traffic away from the SCV along Salmonella-induced-filaments (Sifs). Following infection of RAW cells, SseJ and SifB localize to the SCV as well as LAMP-1-positive, vesicular-appearing structures distant from the SCV. Trafficking of SseJ and SifB away from the SCV requires the SPI-2 effector SifA. Deletion of sseJ, but not sifB, leads to attenuation of Salmonella replication in mice following intraperitoneal inoculation. The contribution of SseJ to in vivo replication is identical in wild-type and sifA deletion backgrounds, suggesting that SseJ trafficking away from the SCV along Sifs is unnecessary for its virulence function.

* Corresponding author. Mailing address: Department of Microbiology, University of Washington, HSB K-140, Box 357710, Seattle, WA 98195. Phone: (206) 616-5107. Fax: (206) 616-4295. E-mail: millersi{at}u.washington.edu.

Editor: B. B. Finlay

{dagger} Present address: Zymogenetics, Seattle, WA 98102.

Infection and Immunity, January 2003, p. 418-427, Vol. 71, No. 1
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.1.418-427.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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