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Infection and Immunity, January 2003, p. 541-545, Vol. 71, No. 1
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.1.541-545.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Campylobacter jejuni Cytolethal Distending Toxin Promotes DNA Repair Responses in Normal Human Cells

Duane C. Hassane, Robert B. Lee, and Carol L. Pickett*

Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, Kentucky 40536

Received 2 May 2002/ Returned for modification 17 June 2002/ Accepted 10 October 2002

Cytolethal distending toxin (CDT) is a multisubunit protein found in various gram-negative bacterial pathogens of humans which is thought to cause cell death by direct DNA damage of host cells. We sought to determine if a cellular response to DNA damage could be detected by exogenous addition of the holotoxin. Exogenous addition of the Campylobacter jejuni 81-176 CDT to primary human fibroblasts resulted in formation of Rad50 foci, which are formed around double-stranded-DNA breaks. Moreover, such foci are formed in both proliferating and nonproliferating cells that are treated with C. jejuni CDT. Fibroblasts that were intoxicated and later stimulated to proliferate failed to divide and remained arrested in the G1 phase of the cell cycle.

* Corresponding author. Mailing address: Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky Medical Center, MS415, 800 Rose St., Lexington, KY 40536-0298. Phone: (859) 323-5313. Fax: (859) 257-8994. E-mail: cpicket{at}uky.edu.

Editor: J. T. Barbieri

Infection and Immunity, January 2003, p. 541-545, Vol. 71, No. 1
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.1.541-545.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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