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Infection and Immunity, January 2003, p. 550-556, Vol. 71, No. 1
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.1.550-556.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

OxyR Acts as a Repressor of Catalase Expression in Neisseria gonorrhoeae

Hsing-Ju Tseng,1 Alastair G. McEwan,1 Michael A. Apicella,2 and Michael P. Jennings1*

Centre for Metals in Biology and Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, The University of Queensland, Brisbane, Queensland 4072, Australia,1 Department of Microbiology, University of Iowa, Iowa City, Iowa 522422

Received 3 June 2002/ Returned for modification 26 June 2002/ Accepted 5 October 2002

It has been reported that Neisseria gonorrhoeae possesses a very high level of catalase activity, but the regulation of catalase expression has not been investigated extensively. In Escherichia coli and Salmonella enterica serovar Typhimurium, it has been demonstrated that OxyR is a positive regulator of hydrogen peroxide-inducible genes, including the gene encoding catalase. The oxyR gene from N. gonorrhoeae was cloned and used to complement an E. coli oxyR mutant, confirming its identity and function. The gene was inactivated by inserting a kanamycin resistance cassette and used to make a knockout allele on the chromosome of N. gonorrhoeae strain 1291. In contrast to E. coli, the N. gonorrhoeae oxyR::kan mutant expressed ninefold-more catalase activity and was more resistant to hydrogen peroxide killing than the wild type. These data are consistent with OxyR in N. gonorrhoeae acting as a repressor of catalase expression.

* Corresponding author. Mailing address: Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, The University of Queensland, Brisbane, QLD 4072, Australia. Phone: 61 7 3365 4879. Fax: 61 7 3365 4620. E-mail: jennings{at}mailbox.uq.edu.au.

Editor: T. R. Kozel

Infection and Immunity, January 2003, p. 550-556, Vol. 71, No. 1
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.1.550-556.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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