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Enhanced Immunogenicity to Mycobacterium tuberculosis by Vaccination with an Alphavirus Plasmid Replicon Expressing Antigen 85A

Joanna R. Kirman,^1, Tara Turon,¹ Hua Su,² Amy Li,³ Carl Kraus,² John M. Polo,⁴ John Belisle,⁵ Sheldon Morris,³ and Robert A. Seder^1*

Cellular Immunology Section, Vaccine Research Center,¹ Laboratory of Immunogenetics,² National Institute of Allergy and Infectious Diseases, National Institutes of Health, and Food and Drug Administration, Bethesda, Maryland,³ Vaccines Research, Chiron Corporation, Emeryville, California,⁴ Microbiology Department, Colorado State University, Fort Collins, Colorado⁵

Received 24 July 2002/ Returned for modification 29 August 2002/ Accepted 10 September 2002

The immunogenicity of a plasmid DNA vaccine incorporating Sindbis virus RNA replicase functions (pSINCP) and expressing antigen 85A (Ag85A) from Mycobacterium tuberculosis was compared with a conventional plasmid DNA vector encoding Ag85A. pSINCP-85A was highly immunogenic in mice and gave enhanced long-term protection against M. tuberculosis compared with the conventional vector.

Editor: T. R. Kozel

Present address: Malaghan Institute of Medical Research, Wellington, New Zealand.

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