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Infection and Immunity, October 2003, p. 6063-6067, Vol. 71, No. 10
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.10.6063-6067.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Sequence and Expression Analysis of virB9 of the Type IV Secretion System of Ehrlichia canis Strains in Ticks, Dogs, and Cultured Cells

Suleyman Felek, Haibin Huang, and Yasuko Rikihisa*

Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio 43210-1093

Received 30 April 2003/ Returned for modification 11 June 2003/ Accepted 23 June 2003

Ehrlichia canis virB9 was cloned and expressed. The sequences of virB9 from six geographic locations were identical. virB9 was transcribed by E. canis in dogs, ticks, and cell culture. Infected dogs had antibodies to recombinant VirB9, indicating that VirB9 was produced by E. canis in dogs and was antigenic.

* Corresponding author. Mailing address: Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, 1925 Coffey Rd., Columbus, OH 43210-1093. Phone: (614) 292-9677. Fax: (614) 292-6473. E-mail: rikihisa.1{at}osu.edu.

Editor: J. T. Barbieri

Infection and Immunity, October 2003, p. 6063-6067, Vol. 71, No. 10
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.10.6063-6067.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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