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Infection and Immunity, October 2003, p. 6068-6074, Vol. 71, No. 10
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.10.6068-6074.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Host Adaptation of Pigeon Isolates of Salmonella enterica subsp. enterica Serovar Typhimurium Variant Copenhagen Phage Type 99 Is Associated with Enhanced Macrophage Cytotoxicity

Frank Pasmans,1* Filip Van Immerseel,1 Marc Heyndrickx,2 An Martel,1 Claudine Godard,3 Christa Wildemauwe,3 Richard Ducatelle,1 and Freddy Haesebrouck1

Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke,1 Center for Agricultural Research, Department of Animal Product Quality and Transformation Technology, 9090 Melle,2 Pasteur Institute of Brussels, Phage Typing Service, 1180 Brussels, Belgium3

Received 21 February 2003/ Returned for modification 1 April 2003/ Accepted 26 June 2003

Phage type 99 of Salmonella enterica subsp. enterica serovar Typhimurium variant Copenhagen strains isolated from pigeons were examined for the presence of genotypic and phenotypic characteristics. The pulsed-field gel electrophoresis patterns obtained with XbaI and BlnI from 38 pigeon strains were compared with those obtained from 89 porcine, poultry, and human strains of variant Copenhagen. Identical patterns with XbaI and four closely related patterns with BlnI were obtained with the pigeon strains, whereas 16 XbaI patterns were found with the other strains. The XbaI patterns of the pigeon strains showed a low genetic similarity to the patterns of the porcine, poultry, and human strains and invariably showed a low-molecular-weight band that was absent in the majority of the other strains. The virulence genes shdA, spvR, pefA, sopE, and spvB were uniformly present in six pigeon isolates representing the genetic diversity found with BlnI. These six pigeon-derived strains were highly cytotoxic for pigeon macrophages compared to three porcine strains. After experimental infection of pigeons with a pigeon strain, clinical symptoms, fecal shedding, and colonization of internal organs were more pronounced than those after infection with a porcine strain. These data suggest that the phage type 99 strains used in this study are highly adapted to pigeons and should be classified as a host-restricted lineage of the serovar Typhimurium.

* Corresponding author. Mailing address: Department of Pathology, Bacteriology and Avain Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium. Phone: 32/9 264 74 44. Fax: 32/9 264 74 94. E-mail: frank.pasmans{at}rug.ac.be.

Editor: B. B. Finlay

Infection and Immunity, October 2003, p. 6068-6074, Vol. 71, No. 10
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.10.6068-6074.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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