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Infection and Immunity, October 2003, p. 6088-6094, Vol. 71, No. 10
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.10.6088-6094.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification and Characterization of a New Staphylococcal Enterotoxin-Related Putative Toxin Encoded by Two Kinds of Plasmids

Katsuhiko Omoe,1* Dong-Liang Hu,2 Hiromi Takahashi-Omoe,3 Akio Nakane,2 and Kunihiro Shinagawa1

Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550,1 Department of Bacteriology, Hirosaki University School of Medicine, Zaifu-cho, Hirosaki 036-8562,2 Department of Technical Support and Development, National Institute of Radiological Sciences, Inage-ku, Chiba-shi 263-8555, Japan3

Received 21 January 2003/ Returned for modification 27 May 2003/ Accepted 23 June 2003

We identified and characterized a novel staphylococcal enterotoxin-like putative toxin, which is named SER. Nucleotide sequencing analysis of the ser gene revealed that ser was most closely related to the seg gene. The ser gene product, SER, was successfully expressed as a recombinant protein in an Escherichia coli expression system, and recombinant SER (rSER) showed significant T-cell stimulation activity. The SER production in ser-harboring Staphylococcus aureus strains was confirmed by Western blot analysis using anti-rSER antibody. Moreover, ser was seen to be encoded by at least two types of plasmids. In particular, one kind of plasmid encoding the ser gene has been known as a sed- and sej-carrying pIB485-related plasmid.

* Corresponding author. Mailing address: Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University, Ueda 3-18-8, Morioka, Iwate 020-8550, Japan. Phone: 81-19-621-6221. Fax: 81-19-621-6223 E-mail: omo{at}iwate-u.ac.jp.

Editor: A. D. O'Brien

Infection and Immunity, October 2003, p. 6088-6094, Vol. 71, No. 10
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.10.6088-6094.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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