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Infection and Immunity, October 2003, p. 6101-6103, Vol. 71, No. 10
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.10.6101-6103.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evaluation of the Roles of Four Candida albicans Genes in Virulence by Using Gene Disruption Strains That Express URA3 from the Native Locus

Departments of Medicine,¹ Molecular Genetics and Microbiology, University of Florida College of Medicine ,³ Center for Molecular Microbiology and Department of Oral Biology, University of Florida College of Dentistry, Gainesville, Florida 32610,⁴ North Florida/South Georgia V.A. Medical Center, Gainesville, Florida 32608²

Received 1 April 2003/ Returned for modification 15 May 2003/ Accepted 9 July 2003

Reintroducing URA3 to its native locus in Candida albicans not5, not3, bur2, and kel1 disruption mutants enabled us to directly compare strains with control strain CAI-12. We showed that URA3 position affected orotidine 5'-monophosphate decarboxylase activity, hyphal morphogenesis, adherence, and mortality in murine disseminated candidiasis. After URA3 was reintroduced to its native locus, only NOT5 could be conclusively ascribed a role in virulence.

Editor: T. R. Kozel

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