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Infection and Immunity, October 2003, p. 6104-6108, Vol. 71, No. 10
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.10.6104-6108.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification of an Inducible Bacteriophage in a Virulent Strain of Streptococcus suis Serotype 2

J. Harel,1,2* G. Martinez,1 A. Nassar,1 H. Dezfulian,1 S. J. Labrie,3 R. Brousseau,2,4 S. Moineau,3 and M. Gottschalk1,2

Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de MontréalC.P. 5000, St-Hyacinthe, Quebec, Canada J2S 7C6,1 Canadian Research Network on Bacterial Pathogens and Swine,2 Biotechnology Research Institute, Montreal,Quebec, Canada H4P 2R2,4 Département de Biochimie et de Microbiologie, Faculté des Sciences et de Génie, Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, Quebec, Canada, G1K 7P43

Received 30 January 2003/ Returned for modification 17 April 2003/ Accepted 21 July 2003

Streptococcus suis infection is considered to be a major problem in the swine industry worldwide. Most virulent Canadian isolates of S. suis serotype 2 do not produce the known virulence markers for this pathogen. PCR-based subtraction hybridization was adapted to isolate unique DNA sequences which were specific to virulent strains of S. suis isolated in Canada. Analysis of some subtracted DNA clones revealed significant homology with bacteriophages of gram-positive bacteria. An inducible phage (named Ss1) was observed in S. suis following the incubation of the virulent strain 89-999 with mitomycin C. Phage Ss1 has a long noncontractile tail and a small isometric nucleocapsid and is a member of the Siphoviridae family. Ss1 phage DNA appears to be present in most Canadian S. suis strains tested in this study, which were isolated from diseased pigs or had proven virulence in mouse or pig models. To our knowledge, this is the first report of the isolation of a phage in S. suis.

* Corresponding author. Mailing address: Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, C.P. 5000, St-Hyacinthe, Quebec J2S 7C6, Canada. Phone: (450) 773-8521, ext. 8233. Fax: (450) 778-8108. E-mail: harelj{at}MEDVET.UMontreal.CA.

Editor: V. J. DiRita

Infection and Immunity, October 2003, p. 6104-6108, Vol. 71, No. 10
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.10.6104-6108.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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