This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Leyton, D. L.
Right arrow Articles by Hartland, E. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Leyton, D. L.
Right arrow Articles by Hartland, E. L.
Right arrowPubmed/NCBI databases
*Domain*Gene
*Genome*Protein
*Substance via MeSH

 Previous Article  |  Next Article 

Infection and Immunity, November 2003, p. 6307-6319, Vol. 71, No. 11
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.11.6307-6319.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Transfer Region of pO113 from Enterohemorrhagic Escherichia coli: Similarity with R64 and Identification of a Novel Plasmid-Encoded Autotransporter, EpeA

Denisse L. Leyton, Joan Sloan, Rebecca E. Hill, Steven Doughty, and Elizabeth L. Hartland*

Bacterial Pathogenesis Research Group, Department of Microbiology, School of Biomedical Sciences, Monash University, Clayton 3800, Victoria, Australia

Received 3 April 2003/ Returned for modification 20 May 2003/ Accepted 15 July 2003

Enterohemorrhagic Escherichia coli (EHEC) is a prominent, food-borne cause of diarrhea, bloody diarrhea, and the hemolytic uremic syndrome in industrialized countries. Most strains of EHEC carry the locus for enterocyte effacement (LEE) pathogenicity island, but a proportion of isolates from patients with severe disease do not carry LEE and very little is known about virulence factors in these organisms. LEE-negative strains of EHEC typically express Shiga toxin 2 and carry a large plasmid that encodes the production of EHEC hemolysin. In this study, we determined the nucleotide sequence of the transfer region of pO113, the large hemolysin plasmid from LEE-negative EHEC O113:H21 (EH41). This 63.9-kb region showed a high degree of similarity with the transfer region of R64, and pO113 was capable of self-transmission at low frequencies. Unlike R64 and the related dot/icm system of Legionella pneumophila, however, pO113 was unable to mobilize RSF1010. In addition, the pO113 transfer region encoded a novel high-molecular-weight serine protease autotransporter of Enterobacteriaceae (SPATE) protein, termed EpeA. Like other SPATEs, EpeA exhibited protease activity and mucinase activity, but expression was not associated with a cytopathic effect on epithelial cells. Analysis of a second high-molecular-weight secreted protein revealed that pO113 also encodes EspP, a cytopathic SPATE identified previously in EHEC O157:H7. The nucleotide sequences encoding the predicted ß-domains of espP and epeA were identical and also shared significant homology with a third SPATE protein, EspI. Both espP and epeA were detected in several LEE-negative clinical isolates of EHEC and thus may contribute to the pathogenesis of this subset of EHEC.

* Corresponding author. Mailing address: Department of Microbiology, Monash University, Clayton 3800, Victoria, Australia. Phone: (61) 3 9905 4323. Fax: (61) 3 9905 4811. E-mail: Liz.Hartland{at}med.monash.edu.au.

Editor: A. D. O'Brien

Infection and Immunity, November 2003, p. 6307-6319, Vol. 71, No. 11
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.11.6307-6319.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Herold, S., Paton, J. C., Paton, A. W. (2009). Sab, a Novel Autotransporter of Locus of Enterocyte Effacement-Negative Shiga-Toxigenic Escherichia coli O113:H21, Contributes to Adherence and Biofilm Formation. Infect. Immun. 77: 3234-3243 [Abstract] [Full Text]  
  • Harrington, S. M., Sheikh, J., Henderson, I. R., Ruiz-Perez, F., Cohen, P. S., Nataro, J. P. (2009). The Pic Protease of Enteroaggregative Escherichia coli Promotes Intestinal Colonization and Growth in the Presence of Mucin. Infect. Immun. 77: 2465-2473 [Abstract] [Full Text]  
  • Cookson, A. L., Bennett, J., Nicol, C., Thomson-Carter, F., Attwood, G. T. (2009). Molecular Subtyping and Distribution of the Serine Protease from Shiga Toxin-Producing Escherichia coli among Atypical Enteropathogenic E. coli Strains. Appl. Environ. Microbiol. 75: 2246-2249 [Abstract] [Full Text]  
  • Bean, D. C., Livermore, D. M., Hall, L. M. C. (2009). Plasmids Imparting Sulfonamide Resistance in Escherichia coli: Implications for Persistence. Antimicrob. Agents Chemother. 53: 1088-1093 [Abstract] [Full Text]  
  • Brockmeyer, J., Bielaszewska, M., Fruth, A., Bonn, M. L., Mellmann, A., Humpf, H.-U., Karch, H. (2007). Subtypes of the Plasmid-Encoded Serine Protease EspP in Shiga Toxin-Producing Escherichia coli: Distribution, Secretion, and Proteolytic Activity. Appl. Environ. Microbiol. 73: 6351-6359 [Abstract] [Full Text]  
  • Leyton, D. L., Adams, L. M., Kelly, M., Sloan, J., Tauschek, M., Robins-Browne, R. M., Hartland, E. L. (2007). Contribution of a Novel Gene, rpeA, Encoding a Putative Autotransporter Adhesin to Intestinal Colonization by Rabbit-Specific Enteropathogenic Escherichia coli. Infect. Immun. 75: 4664-4669 [Abstract] [Full Text]  
  • Dudley, E. G., Abe, C., Ghigo, J.-M., Latour-Lambert, P., Hormazabal, J. C., Nataro, J. P. (2006). An IncI1 Plasmid Contributes to the Adherence of the Atypical Enteroaggregative Escherichia coli Strain C1096 to Cultured Cells and Abiotic Surfaces. Infect. Immun. 74: 2102-2114 [Abstract] [Full Text]  
  • Parham, N. J., Pollard, S. J., Desvaux, M., Scott-Tucker, A., Liu, C., Fivian, A., Henderson, I. R. (2005). Distribution of the Serine Protease Autotransporters of the Enterobacteriaceae among Extraintestinal Clinical Isolates of Escherichia coli. J. Clin. Microbiol. 43: 4076-4082 [Abstract] [Full Text]  
  • Luck, S. N., Bennett-Wood, V., Poon, R., Robins-Browne, R. M., Hartland, E. L. (2005). Invasion of Epithelial Cells by Locus of Enterocyte Effacement-Negative Enterohemorrhagic Escherichia coli. Infect. Immun. 73: 3063-3071 [Abstract] [Full Text]  
  • Henderson, I. R., Navarro-Garcia, F., Desvaux, M., Fernandez, R. C., Ala'Aldeen, D. (2004). Type V Protein Secretion Pathway: the Autotransporter Story. Microbiol. Mol. Biol. Rev. 68: 692-744 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»