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Infection and Immunity, November 2003, p. 6420, Vol. 71, No. 11
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.11.6420-6425.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Modulation of the Bovine Delayed-Type Hypersensitivity Responses to Defined Mycobacterial Antigens by a Synthetic Bacterial Lipopeptide

A. O. Whelan,1* J. C. Hope,2 C. J. Howard,2 D. Clifford,1 R. G. Hewinson,1 and H. M. Vordermeier1

TB Research Group, Veterinary Laboratories Agency-Weybridge, New Haw, Addlestone, Surrey,1 Institute for Animal Health, Compton, Newbury, Berkshire, United Kingdom2

Received 14 January 2003/ Returned for modification 26 March 2003/ Accepted 10 July 2003

The use of defined protein and peptide antigens can overcome specificity limitations of purified protein derivatives in the detection of bovine tuberculosis when the antigens are used in blood-based tests. Since the use of these specific antigens as skin test reagents could have practical advantages, we investigated the potential of Mycobacterium bovis-specific antigens to stimulate delayed-type hypersensitivity (DTH) responses in cattle experimentally infected with M. bovis. A cocktail of the recombinant antigens ESAT-6, MPB83, and MPB64 failed to stimulate in vivo DTH in cattle that had been experimentally infected with M. bovis despite the fact that the antigens were recognized in vitro by the same animals. However, it was possible to stimulate antigen-specific bovine DTH responses by using ESAT-6 in combination with a synthetic bacterial lipopeptide. This lipopeptide stimulated the release of the proinflammatory cytokine tumor necrosis factor alpha from monocyte-derived bovine dendritic cells in vitro, thereby providing a possible mechanism for its DTH-enhancing properties.

* Corresponding author. Mailing address: VLA-Weybridge, Woodham Lane, New Haw, Addlestone, Surrey, KT15 3NB, United Kingdom. Phone: 44 (0)1932 357506. Fax: 44 (0)1932 357684. E-mail: a.o.whelan{at}vla.defra.gsi.gov.uk.

Editor: S. H. E. Kaufmann

Infection and Immunity, November 2003, p. 6420, Vol. 71, No. 11
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.11.6420-6425.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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