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Infection and Immunity, November 2003, p. 6627-6632, Vol. 71, No. 11
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.11.6627-6632.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Simultaneous Variation of the Immunodominant Outer Membrane Proteins, MSP2 and MSP3, during Anaplasma marginale Persistence In Vivo

Kelly A. Brayton,1* Patrick F. M. Meeus,2 Anthony F. Barbet,2 and Guy H. Palmer1

Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040,1 Department of Pathobiology, College of Veterinary Medicine, University of Florida, Gainesville, Florida 32611-08802

Received 28 March 2003/ Returned for modification 18 June 2003/ Accepted 11 August 2003

Vector-borne bacterial pathogens persist in the mammalian host by varying surface antigens to evade the existing immune response. To test whether the model of surface coat switching and immune evasion can be extended to a vector-borne bacterial pathogen with multiple immunodominant surface proteins, we examined Anaplasma marginale, a rickettsia with two highly immunogenic outer membrane proteins, major surface protein 2 (MSP2) and MSP3. The simultaneous clearance of variants of the two most immunodominant surface proteins of A. marginale followed by emergence of unique variants indicates that the switch rates and immune selection for MSP2 and MSP3 are sufficiently similar to explain the cyclic bacteremia observed during infection in the immunocompetent host.

* Corresponding author. Mailing address: Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040. Phone: (509) 335-6340. Fax: (509) 335-8529. E-mail: kbrayton{at}vetmed.wsu.edu.

Infection and Immunity, November 2003, p. 6627-6632, Vol. 71, No. 11
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.11.6627-6632.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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