This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Adderson, E. E.
Right arrow Articles by Bohnsack, J. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Adderson, E. E.
Right arrow Articles by Bohnsack, J. F.

 Previous Article  |  Next Article 

Infection and Immunity, December 2003, p. 6857-6863, Vol. 71, No. 12
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.12.6857-6863.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Subtractive Hybridization Identifies a Novel Predicted Protein Mediating Epithelial Cell Invasion by Virulent Serotype III Group B Streptococcus agalactiae

Elisabeth E. Adderson,1* Shinji Takahashi,2 Yan Wang,1 Jianling Armstrong,1 Dylan V. Miller,3 and John F. Bohnsack3,4

Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38105,1 Division of Microbiology, Joshi-Eiyoh University, Chiyoda, Saitama 350-0288, Japan,2 Departments of Pediatrics,3 Pathology,University of Utah School of Medicine, Salt Lake City, Utah 841324

Received 5 December 2002/ Returned for modification 8 May 2003/ Accepted 28 August 2003

Group B Streptococcus agalactiae bacteria (group B streptococci [GBS]) are the most common cause of serious bacterial infection in newborn infants. The majority of serotype III-related cases of neonatal disease are caused by a genetically related subgroup of bacteria, restriction fragment digest pattern (RDP) type III-3, suggesting that these strains possess unique genes contributing to virulence. We used genomic subtractive hybridization to identify regions of genomic DNA unique to virulent RDP type III-3 GBS strains. Within one of these III-3-specific regions is a 1,506-bp open reading frame, spb1 (surface protein of group B streptococcus 1). A mutant type III GBS strain lacking Spb1 was constructed in virulent RDP type III-3 strain 874391, and the interactions of the wild-type and spb1 isogenic mutant with a variety of epithelial cells important to GBS colonization and infection were compared. While adherence of the spb1 isogenic mutant to A549 respiratory, C2Bbe1 colonic, and HeLa cervical epithelial cells was slightly lower than that of the 874391 strain, invasion of the Spb1- mutant was significantly reduced with these cell lines compared to what was seen with 874391. The defect in epithelial invasion was corrected by supplying spb1 in trans. These observations suggest that Spb1 contributes to the pathogenesis of neonatal GBS infection by mediating internalization of virulent serotype III GBS and confirm that understanding of the population structure of bacteria may lead to insights into the pathogenesis of human infections.

* Corresponding author. Mailing address: Rm. E8054, Mailstop 320, St. Jude Children's Research Hospital, 332 N. Lauderdale St., Memphis, TN 38105. Phone: (901) 495-3459. Fax: (901) 495-3099. E-mail: Elisabeth.Adderson{at}stjude.org.

Editor: V. J. DiRita

Infection and Immunity, December 2003, p. 6857-6863, Vol. 71, No. 12
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.12.6857-6863.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nobbs, A. H., Lamont, R. J., Jenkinson, H. F. (2009). Streptococcus Adherence and Colonization. Microbiol. Mol. Biol. Rev. 73: 407-450 [Abstract] [Full Text]  
  • Baron, M. J., Filman, D. J., Prophete, G. A., Hogle, J. M., Madoff, L. C. (2007). Identification of a Glycosaminoglycan Binding Region of the Alpha C Protein That Mediates Entry of Group B Streptococci into Host Cells. J. Biol. Chem. 282: 10526-10536 [Abstract] [Full Text]  
  • Seifert, K. N., Adderson, E. E., Whiting, A. A., Bohnsack, J. F., Crowley, P. J., Brady, L. J. (2006). A unique serine-rich repeat protein (Srr-2) and novel surface antigen ({varepsilon}) associated with a virulent lineage of serotype III Streptococcus agalactiae.. Microbiology 152: 1029-1040 [Abstract] [Full Text]  
  • Whitby, P. W., VanWagoner, T. M., Taylor, A. A., Seale, T. W., Morton, D. J., LiPuma, J. J., Stull, T. L. (2006). Identification of an RTX determinant of Burkholderia cenocepacia J2315 by subtractive hybridization. J Med Microbiol 55: 11-21 [Abstract] [Full Text]  
  • Tettelin, H., Masignani, V., Cieslewicz, M. J., Donati, C., Medini, D., Ward, N. L., Angiuoli, S. V., Crabtree, J., Jones, A. L., Durkin, A. S., DeBoy, R. T., Davidsen, T. M., Mora, M., Scarselli, M., Margarit y Ros, I., Peterson, J. D., Hauser, C. R., Sundaram, J. P., Nelson, W. C., Madupu, R., Brinkac, L. M., Dodson, R. J., Rosovitz, M. J., Sullivan, S. A., Daugherty, S. C., Haft, D. H., Selengut, J., Gwinn, M. L., Zhou, L., Zafar, N., Khouri, H., Radune, D., Dimitrov, G., Watkins, K., O'Connor, K. J. B., Smith, S., Utterback, T. R., White, O., Rubens, C. E., Grandi, G., Madoff, L. C., Kasper, D. L., Telford, J. L., Wessels, M. R., Rappuoli, R., Fraser, C. M. (2005). Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae: Implications for the microbial "pan-genome". Proc. Natl. Acad. Sci. USA 102: 13950-13955 [Abstract] [Full Text]  
  • Luan, S.-L., Granlund, M., Sellin, M., Lagergard, T., Spratt, B. G., Norgren, M. (2005). Multilocus Sequence Typing of Swedish Invasive Group B Streptococcus Isolates Indicates a Neonatally Associated Genetic Lineage and Capsule Switching. J. Clin. Microbiol. 43: 3727-3733 [Abstract] [Full Text]  
  • Lindahl, G., Stalhammar-Carlemalm, M., Areschoug, T. (2005). Surface Proteins of Streptococcus agalactiae and Related Proteins in Other Bacterial Pathogens. Clin. Microbiol. Rev. 18: 102-127 [Abstract] [Full Text]  
  • Aoyagi, Y., Adderson, E. E., Min, J. G., Matsushita, M., Fujita, T., Takahashi, S., Okuwaki, Y., Bohnsack, J. F. (2005). Role of L-Ficolin/Mannose-Binding Lectin-Associated Serine Protease Complexes in the Opsonophagocytosis of Type III Group B Streptococci. J. Immunol. 174: 418-425 [Abstract] [Full Text]  
  • Schubert, A., Zakikhany, K., Pietrocola, G., Meinke, A., Speziale, P., Eikmanns, B. J., Reinscheid, D. J. (2004). The Fibrinogen Receptor FbsA Promotes Adherence of Streptococcus agalactiae to Human Epithelial Cells. Infect. Immun. 72: 6197-6205 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»