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Infection and Immunity, December 2003, p. 7035-7042, Vol. 71, No. 12
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.12.7035-7042.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Effect of Mycobacterium bovis BCG Vaccination on Mycobacterium-Specific Cellular Proliferation and Tumor Necrosis Factor Alpha Production from Distinct Guinea Pig Leukocyte Populations

Todd M. Lasco,1* Toshiko Yamamoto,1 Teizo Yoshimura,2 Shannon Sedberry Allen,1 Lynne Cassone,3 and David N. McMurray1

Department of Medical Microbiology and Immunology, The Texas A&M University System-Health Science Center,1 College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843,3 Laboratory of Molecular Immunoregulation, National Cancer Institute,Frederick, Maryland 217022

Received 5 May 2003/ Returned for modification 2 July 2003/ Accepted 2 September 2003

In this study, we focused on three leukocyte-rich guinea pig cell populations, bronchoalveolar lavage (BAL) cells, resident peritoneal cells (PC), and splenocytes (SPC). BAL cells, SPC, and PC were stimulated either with live attenuated Mycobacterium tuberculosis H37Ra or with live or heat-killed virulent M. tuberculosis H37Rv (multiplicity of infection of 1:100). Each cell population was determined to proliferate in response to heat-killed virulent H37Rv, whereas no measurable proliferative response could be detected upon stimulation with live mycobacteria. Additionally, this proliferative capacity (in SPC and PC populations) was significantly enhanced upon prior vaccination with Mycobacterium bovis BCG. Accordingly, in a parallel set of experiments we found a strong positive correlation between production of antigen-specific bioactive tumor necrosis factor alpha (TNF-{alpha}) and prior vaccination with BCG. A nonspecific stimulus, lipopolysaccharide, failed to induce this effect on BAL cells, SPC, and PC. These results showed that production of bioactive TNF-{alpha} from mycobacterium-stimulated guinea pig cell cultures positively correlates with the vaccination status of the host and with the virulence of the mycobacterial strain.

* Corresponding author. Mailing address: Department of Microbiology, Immunology, and Pathology, Colorado State University, 320B Microbiology Building (1682 Campus Delivery), Fort Collins, CO 80523-1682. Phone: (970) 491-7469. Fax: (970) 491-5125. E-mail: Todd.Lasco{at}colostate.edu.

Editor: S. H. E. Kaufmann

Infection and Immunity, December 2003, p. 7035-7042, Vol. 71, No. 12
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.12.7035-7042.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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