This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dalle, F. Articles by Bonnin, A. Search for Related Content PubMed PubMed Citation Articles by Dalle, F. Articles by Bonnin, A.

 Previous Article  |  Next Article 

Infection and Immunity, December 2003, p. 7061-7068, Vol. 71, No. 12
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.12.7061-7068.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

ß-1,2- and -1,2-Linked Oligomannosides Mediate Adherence of Candida albicans Blastospores to Human Enterocytes In Vitro

Laboratoire de Parasitologie Mycologie,¹ Service d'Informatique Médicale et Biostatistique, Hôpital du Bocage,⁵ Laboratoire de Microbiologie Médicale et Moléculaire, Faculté de Médecine, 21079 Dijon Cedex,² Laboratoire de Mycologie Fondamentale et Appliquée, Equipe INSERM 0360, Faculté de Médecine Pôle Recherche, 59037 Lille Cedex,³ Département de Chimie, Ecole Normale Supérieure, UMR CNRS 8642, 75231 Paris Cedex 05, France⁴

Received 12 May 2003/ Returned for modification 22 June 2003/ Accepted 2 August 2003

Candida albicans is a commensal dimorphic yeast of the digestive tract that causes hematogenously disseminated infections in immunocompromised individuals. Endogenous invasive candidiasis develops from C. albicans adhering to the intestinal epithelium. Adherence is mediated by the cell wall surface, a domain composed essentially of mannopyranosyl residues bound to proteins, the N-linked moiety of which comprises sequences of -1,2- and ß-1,2-linked mannose residues. ß-1,2-linked mannosides are also associated with a glycolipid, phospholipomannan, at the C. albicans surface. In order to determine the roles of ß-1,2 and -1,2 oligomannosides in the C. albicans-enterocyte interaction, we developed a model of adhesion of C. albicans VW32 blastospores to the apical regions of differentiated Caco-2 cells. Preincubation of yeasts with monoclonal antibodies (MAbs) specific for -1,2 and ß-1,2 mannan epitopes resulted in a dose-dependent decrease in adhesion (50% of the control with a 60-µg/ml MAb concentration). In competitive assays ß-1,2 and -1,2 tetramannosides were the most potent carbohydrate inhibitors, with 50% inhibitory concentrations of 2.58 and 6.99 mM, respectively. Immunolocalization on infected monolayers with MAbs specific for -1,2 and ß-1,2 oligomannosides showed that these epitopes were shed from the yeast to the enterocyte surface. Taken together, our data indicate that -1,2 and ß-1,2 oligomannosides are involved in the C. albicans-enterocyte interaction and participate in the adhesion of the yeasts to the mucosal surface.

Editor: T. R. Kozel

This article has been cited by other articles:

• Zhang, M. X., Bohlman, M. C., Itatani, C., Burton, D. R., Parren, P. W. H. I., St. Jeor, S. C., Kozel, T. R. (2006). Human Recombinant Antimannan Immunoglobulin G1 Antibody Confers Resistance to Hematogenously Disseminated Candidiasis in Mice. Infect. Immun. 74: 362-369 [Abstract] [Full Text]  
• Pretzer, G., Snel, J., Molenaar, D., Wiersma, A., Bron, P. A., Lambert, J., de Vos, W. M., van der Meer, R., Smits, M. A., Kleerebezem, M. (2005). Biodiversity-Based Identification and Functional Characterization of the Mannose-Specific Adhesin of Lactobacillus plantarum. J. Bacteriol. 187: 6128-6136 [Abstract] [Full Text]  
• Rouabhia, M., Schaller, M., Corbucci, C., Vecchiarelli, A., Prill, S. K.-H., Giasson, L., Ernst, J. F. (2005). Virulence of the Fungal Pathogen Candida albicans Requires the Five Isoforms of Protein Mannosyltransferases. Infect. Immun. 73: 4571-4580 [Abstract] [Full Text]  
• Brun, S., Dalle, F., Saulnier, P., Renier, G., Bonnin, A., Chabasse, D., Bouchara, J.-P. (2005). Biological consequences of petite mutations in Candida glabrata. J Antimicrob Chemother 56: 307-314 [Abstract] [Full Text]  
• Singleton, D. R., Masuoka, J., Hazen, K. C. (2005). Surface Hydrophobicity Changes of Two Candida albicans Serotype B mnn4{Delta} Mutants. Eukaryot Cell 4: 639-648 [Abstract] [Full Text]  
• Munro, C. A., Bates, S., Buurman, E. T., Hughes, H. B., MacCallum, D. M., Bertram, G., Atrih, A., Ferguson, M. A. J., Bain, J. M., Brand, A., Hamilton, S., Westwater, C., Thomson, L. M., Brown, A. J. P., Odds, F. C., Gow, N. A. R. (2005). Mnt1p and Mnt2p of Candida albicans Are Partially Redundant {alpha}-1,2-Mannosyltransferases That Participate in O-Linked Mannosylation and Are Required for Adhesion and Virulence. J. Biol. Chem. 280: 1051-1060 [Abstract] [Full Text]  
• Mille, C., Janbon, G., Delplace, F., Ibata-Ombetta, S., Gaillardin, C., Strecker, G., Jouault, T., Trinel, P.-A., Poulain, D. (2004). Inactivation of CaMIT1 Inhibits Candida albicans Phospholipomannan {beta}-Mannosylation, Reduces Virulence, and Alters Cell Wall Protein {beta}-Mannosylation. J. Biol. Chem. 279: 47952-47960 [Abstract] [Full Text]  
• Hobson, R. P., Munro, C. A., Bates, S., MacCallum, D. M., Cutler, J. E., Heinsbroek, S. E. M., Brown, G. D., Odds, F. C., Gow, N. A.R. (2004). Loss of Cell Wall Mannosylphosphate in Candida albicans Does Not Influence Macrophage Recognition. J. Biol. Chem. 279: 39628-39635 [Abstract] [Full Text]