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Infection and Immunity, December 2003, p. 7140-7148, Vol. 71, No. 12
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.12.7140-7148.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Role for Complement in Development of Helicobacter-Induced Gastritis in Interleukin-10-Deficient Mice

Hanan F. Ismail,1 Juan Zhang,1 Richard G. Lynch,2 Yi Wang,3 and Daniel J. Berg1*

Department of Internal Medicine,1 Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa 52242,2 Alexion Pharmaceuticals, Inc., Cheshire, Connecticut 064103

Received 12 March 2003/ Returned for modification 7 May 2003/ Accepted 11 August 2003

The mechanisms by which the immune response can eradicate gastric Helicobacter infection are unknown. We hypothesized that Helicobacter-induced activation of the complement system could promote both inflammation and eradication of Helicobacter from the stomach. In vitro studies demonstrated that Helicobacter felis activates complement in normal mouse serum but not in serum from Rag2-/- mice, indicating that H. felis activates complement through the classical pathway. Next, we infected complement-depleted wild-type control and interleukin-10-deficient (IL-10-/-) mice with H. felis. Helicobacter infection of wild-type mice elicited a mild, focal gastritis and did not alter serum complement levels. Infection of IL-10-/- mice with H. felis elicited severe gastritis. After the initial colonization, the IL-10-/- mice completely cleared Helicobacter from the stomach by day 8. In contrast to wild-type mice, H. felis-infected IL-10-/- mice had a marked increase in serum complement levels. Complement depletion of wild-type mice did not affect the intensity of gastric inflammation or the extent of Helicobacter colonization compared to that for the wild-type control mice. In contrast, complement depletion of Helicobacter-infected IL-10-/- mice decreased the severity of gastritis, decreased the Helicobacter-induced infiltration of neutrophils into the stomach, and delayed the clearance of bacteria. In vitro studies of stimulated splenocytes and neutrophils from IL-10-/- mice produced a twofold increase in complement production compared to that for wild-type mice. Pretreatment with IL-10 inhibited this increase. These studies identify a role for complement in the local immune response to gastric Helicobacter in IL-10-/- mice and suggest a role for IL-10 in the regulation of complement production.

* Corresponding author. Mailing address: Department of Internal Medicine, C32-GH, University of Iowa Hospitals, 200 Hawkins Drive, Iowa City, IA 52242. Phone: (319) 353-7800. Fax: (319) 353-8383. E-mail: daniel-j-berg{at}uiowa.edu.

Editor: T. R. Kozel

Infection and Immunity, December 2003, p. 7140-7148, Vol. 71, No. 12
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.12.7140-7148.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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