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Infection and Immunity, December 2003, p. 7164-7169, Vol. 71, No. 12
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.12.7164-7169.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The Siderophore Receptor IroN of Extraintestinal Pathogenic Escherichia coli Is a Potential Vaccine Candidate

Thomas A. Russo,1,2,3,4* Catherine D. McFadden,1,3 Ulrike B. Carlino-MacDonald,1,3 Janet M. Beanan,1,3 Ruth Olson,1,3 and Gregory E. Wilding5

Departmentof Medicine,1 Department of Microbiology,2 Witebsky Center for Microbial Pathogenesis,3 VA MedicalCenter,4 Department of Biostatistics, University at Buffalo, Buffalo, New York 142145

Received 27 May 2003/ Returned for modification 15 August 2003/ Accepted 5 September 2003

It would be medically and economically desirable to prevent the millions of annual extraintestinal infections and the thousands of associated deaths due to Escherichia coli. Outer membrane proteins are potential vaccine candidates for the prevention of these infections. This study tested the hypotheses that the siderophore receptor IroN is antigenic and that an IroN-specific antibody response confers protection in vivo. Subcutaneous immunization with denatured IroN resulted in a significant IroN immunoglobulin G (IgG)-specific response in serum (P < 0.0001) but not a systemic or mucosal IroN-specific IgA response. In a mouse model of ascending urinary tract infection, subcutaneous immunization with denatured IroN conferred significant protection against renal (P = 0.0135 and 0.0095 in two independent experiments), but not bladder, infection. These data, together with the previously demonstrated role of IroN in virulence, its expression in human biologic fluids, and its prevalence among extraintestinal pathogenic E. coli strains, support further studies on the role of IroN as a vaccine candidate.

* Corresponding author. Mailing address: Department of Medicine, Division of Infectious Diseases, 3435 Main St., Biomedical Research Building, Room 141, Buffalo, NY 14214. Phone: (716) 829-2674. Fax: (716) 829-3889. E-mail: trusso{at}acsu.buffalo.edu.

Editor: J. T. Barbieri

Infection and Immunity, December 2003, p. 7164-7169, Vol. 71, No. 12
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.12.7164-7169.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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