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Infection and Immunity, December 2003, p. 7188-7192, Vol. 71, No. 12
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.12.7188-7192.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Role of Urease Enzymes in Stability of a 10-Species Oral Biofilm Consortium Cultivated in a Constant-Depth Film Fermenter

Man Shu,{dagger} Christopher M. Browngardt, Yi-Ywan M. Chen, and Robert A. Burne*

Department of Oral Biology, University of Florida College of Dentistry, Gainesville, Florida 32610-0424

Received 24 July 2003/ Returned for modification 30 August 2003/ Accepted 11 September 2003

Using a 10-species oral biofilm consortium and defined mutants, we show that high-level capacity to generate ammonia from a common salivary substrate is needed to maintain community diversity. This model appears to be suitable for the study of the effects of individual genetic determinants on the ecology of oral biofilms.


* Corresponding author. Mailing address: Department of Oral Biology, University of Florida College of Dentistry, 1600 SW Archer Rd., Gainesville, FL 32610-0424. Phone: (352) 392-4370. Fax: (352) 392-7357. E-mail: rburne{at}dental.ufl.edu.

Editor: V. J. DiRita

{dagger} Present address: College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, FL 33328.


Infection and Immunity, December 2003, p. 7188-7192, Vol. 71, No. 12
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.12.7188-7192.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.




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