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Infection and Immunity, December 2003, p. 7202-7207, Vol. 71, No. 12
0019-9567/03/$08.00+0 DOI: 10.1128/IAI.71.12.7202-7207.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
Genetic Analysis of the Capsule Locus of Haemophilus influenzae Serotype f
Sarah W. Satola, Patricia L. Schirmer, and Monica M. Farley*
Atlanta
Veterans Affairs Medical Center and Department of Medicine, Emory
University School of Medicine, Decatur, Georgia
30033
Received 19 June 2003/
Returned for modification 18 July 2003/
Accepted 3 September 2003
A
19-kb DNA region containing genes involved in the biosynthesis of the
capsule of Haemophilus influenzae serotype f (Hif) has been
cloned and characterized. The Hif cap locus organization is
typical of group II capsule biosynthetic loci found in other H.
influenzae serotype b bacteria and other gram-negative bacteria.
However, the Hif cap locus was not associated with an
IS1016 element. Three new open reading frames, Fcs1, Fcs2, and
Fcs3, were identified in the Hif capsule-specific region II. The
chromosomal location of the Hif cap locus and the organization
of the flanking sequences differed significantly from previously
described division I H. influenzae
serotypes.
* Corresponding
author. Mailing address: Atlanta VA Medical Center, Medical Research
Service 151, 1670 Clairmont Rd., Decatur, GA 30033. Phone: (404)
728-7688. Fax: (404) 329-2210. E-mail:
mfarley{at}emory.edu.
Editor:
J. N. Weiser
Infection and Immunity, December 2003, p. 7202-7207, Vol. 71, No. 12
0019-9567/03/$08.00+0 DOI: 10.1128/IAI.71.12.7202-7207.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
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