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Infection and Immunity, December 2003, p. 7208-7210, Vol. 71, No. 12
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.12.7208-7210.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Functional BvgAS Virulence Control System in Bordetella bronchiseptica Is Necessary for Induction of Ca²⁺ Transients in Ciliated Tracheal Epithelial Cells

Department of Zoology and Physiology and Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82701,¹ Mycobacteria Research Laboratories, Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523-1682,² Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840,³ Department of Physiology, Arizona Respiratory Center, University of Arizona, Tucson, Arizona 85724-5030⁴

Received 18 August 2003/ Returned for modification 2 September 2003/ Accepted 9 September 2003

To study initial Bordetella bronchiseptica-tracheal epithelial cell interactions, we coincubated B. bronchiseptica with rabbit tracheal explant cultures and assayed bacterial adherence and host cell Ca²⁺ signaling. Wild-type B. bronchiseptica (RB50) preferentially adhered to cilia and induced ciliated host cell Ca²⁺ transients within 2 min of coincubation, whereas coincubation with an avirulent strain (RB57) resulted in limited binding and Ca²⁺ signaling. The described cell system allows for assessment of initial B. bronchiseptica-host cell interactions that can contribute to pathogenicity or to host cell defense.

Editor: D. L. Burns

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