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Infection and Immunity, February 2003, p. 1011-1015, Vol. 71, No. 2
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.2.1011-1015.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Modulation of J774.1 Macrophage L-Arginine Metabolism by Intracellular Mycobacterium bovis BCG

Marcy A. Peteroy-Kelly,^1,2,3, Vishwanath Venketaraman,^1,2,3,4 Meliza Talaue,^1,2,3 Anjali Seth,^1,2,3 and Nancy D. Connell^1,2,3,4*

Department of Microbiology and Molecular Genetics,¹ National Tuberculosis Center, Department of Medicine,² Center for Emerging and Reemerging Pathogens,³ International Center for Public Health, UMDNJ/New Jersey Medical School, Newark, New Jersey 07101-1709⁴

Received 30 January 2002/ Returned for modification 26 March 2002/ Accepted 7 November 2002

Using a Mycobacterium bovis BCG mutant (AS1) lacking a Bacillus subtilis L-arginine transporter homolog, we demonstrate here that the interaction between intracellular mycobacteria and the macrophage with respect to L-arginine transport and metabolism is quite complex. Intracellular AS1 stimulates macrophage L-arginine transport and accumulates 2.5-fold more ³H label derived from L-arginine than does the wild type. These studies suggest that the accumulation of ³H label reflects the acquisition of metabolites of L-arginine produced by the macrophage.

Editor: S. H. E. Kaufmann

Present address: School of Theoretical and Applied Sciences, Ramapo College of New Jersey, Mahwah, NJ 07430.

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