This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chattopadhyay, R.
Right arrow Articles by Chitnis, C. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chattopadhyay, R.
Right arrow Articles by Chitnis, C. E.

 Previous Article  |  Next Article 

Infection and Immunity, February 2003, p. 597-604, Vol. 71, No. 2
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.2.597-604.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Plasmodium falciparum Infection Elicits Both Variant-Specific and Cross-Reactive Antibodies against Variant Surface Antigens

Rana Chattopadhyay,1,{dagger} Amit Sharma,1 Vinod K. Srivastava,2 Sudhanshu S. Pati,2 S. K. Sharma,3 Bhabani S. Das,2 and Chetan E. Chitnis1*

Malaria Research Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi,1 Department of Biochemistry, Ispat General Hospital (IGH),2 Malaria Research Centre Field Station, Rourkela, India3

Received 2 January 2002/ Returned for modification 25 February 2002/ Accepted 12 June 2002

Naturally acquired antibodies to Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP-1), the variant surface antigens expressed on the surface of infected erythrocytes, are thought to play a role in protection against P. falciparum malaria. Here, we have studied the development of antibodies to PfEMP-1 in adult malaria patients living in Rourkela, India, an area with a low malaria transmission rate, and prevalence of antibodies to PfEMP-1 in residents of San Dulakudar, India, a village in which P. falciparum malaria is hyperendemic. Convalescent-phase sera from adult malaria patients from Rourkela agglutinate homologous P. falciparum isolates as well as some heterologous isolates, suggesting that they develop partially cross-reactive antibodies to PfEMP-1 following infection. Adult sera from San Dulakudar agglutinate diverse P. falciparum isolates, suggesting that they have antibodies with wide recognition of diverse PfEMP-1. Mixed-agglutination assays using pairs of P. falciparum isolates confirm the presence of both variant-specific and partially cross-reactive antibodies in convalescent-phase sera from Rourkela and adult sera from San Dulakudar. Analysis of PfEMP-1 sequences suggests a molecular basis for the observed cross-reactivity.


* Corresponding author. Mailing address: Malaria Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), P.O. Box 10504, Aruna Asaf Ali Marg, New Delhi 110067, India. Phone: 91 11 618 7695. Fax: 91 11 616 2316. E-mail: cchitnis{at}icgeb.res.in.

Editor: S. H. E. Kaufmann

{dagger} Present address: Naval Medical Research Centre, Silver Spring, MD 20910-7500.


Infection and Immunity, February 2003, p. 597-604, Vol. 71, No. 2
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.2.597-604.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.




This article has been cited by other articles:

  • Mayor, A., Rovira-Vallbona, E., Srivastava, A., Sharma, S. K., Pati, S. S., Puyol, L., Quinto, L., Bassat, Q., Machevo, S., Mandomando, I., Chauhan, V. S., Alonso, P. L., Chitnis, C. E. (2009). Functional and Immunological Characterization of a Duffy Binding-Like Alpha Domain from Plasmodium falciparum Erythrocyte Membrane Protein 1 That Mediates Rosetting. Infect. Immun. 77: 3857-3863 [Abstract] [Full Text]  
  • Buckee, C. O, Bull, P. C, Gupta, S. (2009). Inferring malaria parasite population structure from serological networks. Proc R Soc B 276: 477-485 [Abstract] [Full Text]  
  • Doolan, D. L., Dobano, C., Baird, J. K. (2009). Acquired Immunity to Malaria. Clin. Microbiol. Rev. 22: 13-36 [Abstract] [Full Text]  
  • Kyes, S. A., Kraemer, S. M., Smith, J. D. (2007). Antigenic Variation in Plasmodium falciparum: Gene Organization and Regulation of the var Multigene Family. Eukaryot Cell 6: 1511-1520 [Full Text]  
  • Moll, K., Pettersson, F., Vogt, A. M., Jonsson, C., Rasti, N., Ahuja, S., Spangberg, M., Mercereau-Puijalon, O., Arnot, D. E., Wahlgren, M., Chen, Q. (2007). Generation of Cross-Protective Antibodies against Plasmodium falciparum Sequestration by Immunization with an Erythrocyte Membrane Protein 1-Duffy Binding-Like 1{alpha} Domain. Infect. Immun. 75: 211-219 [Abstract] [Full Text]  
  • Yone, C. L. R. P., Kremsner, P. G., Luty, A. J. F. (2005). Immunoglobulin G Isotype Responses to Erythrocyte Surface-Expressed Variant Antigens of Plasmodium falciparum Predict Protection from Malaria in African Children. Infect. Immun. 73: 2281-2287 [Abstract] [Full Text]  
  • Gatton, M. L., Cheng, Q. (2004). Modeling the Development of Acquired Clinical Immunity to Plasmodium falciparum Malaria. Infect. Immun. 72: 6538-6545 [Abstract] [Full Text]  
  • SHARMA, S. K., CHATTOPADHYAY, R., CHAKRABARTI, K., PATI, S. S., SRIVASTAVA, V. K., TYAGI, P. K., MAHANTY, S., MISRA, S. K., ADAK, T., DAS, B. S., CHITNIS, C. E. (2004). EPIDEMIOLOGY OF MALARIA TRANSMISSION AND DEVELOPMENT OF NATURAL IMMUNITY IN A MALARIA-ENDEMIC VILLAGE, SAN DULAKUDAR, IN ORISSA STATE, INDIA. Am J Trop Med Hyg 71: 457-465 [Abstract] [Full Text]  
  • ABDEL-LATIF, M. S., CABRERA, G., KOHLER, C., KREMSNER, P. G., LUTY, A. J. F. (2004). ANTIBODIES TO RIFIN: A COMPONENT OF NATURALLY ACQUIRED RESPONSES TO PLASMODIUM FALCIPARUM VARIANT SURFACE ANTIGENS ON INFECTED ERYTHROCYTES. Am J Trop Med Hyg 71: 179-186 [Abstract] [Full Text]  
  • Cabrera, G., Yone, C., Tebo, A. E., van Aaken, J., Lell, B., Kremsner, P. G., Luty, A. J. F. (2004). Immunoglobulin G Isotype Responses to Variant Surface Antigens of Plasmodium falciparum in Healthy Gabonese Adults and Children during and after Successive Malaria Attacks. Infect. Immun. 72: 284-294 [Abstract] [Full Text]  
  • Hviid, L., Staalsoe, T., Nielsen, M. A., Theander, T. G. (2003). Specificity and Cross-Reactivity of Plasmodium falciparum Variant Surface Antigen-Specific Antibody Responses. Infect. Immun. 71: 2296-2296 [Full Text]  
  • Roberts, D. J. (2003). Understanding Naturally Acquired Immunity to Plasmodium falciparum Malaria. Infect. Immun. 71: 589-590 [Full Text]