This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kingsley, R. A.
Right arrow Articles by Bäumler, A. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kingsley, R. A.
Right arrow Articles by Bäumler, A. J.

 Previous Article  |  Next Article 

Infection and Immunity, February 2003, p. 629-640, Vol. 71, No. 2
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.2.629-640.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular and Phenotypic Analysis of the CS54 Island of Salmonella enterica Serotype Typhimurium: Identification of Intestinal Colonization and Persistence Determinants

Robert A. Kingsley,1 Andrea D. Humphries,1 Eric H. Weening,1 Marcel R. de Zoete,1 Sebastian Winter,1 Anastasia Papaconstantinopoulou,2 Gordon Dougan,2 and Andreas J. Bäumler1*

Department of Medical Microbiology and Immunology, College of Medicine, Texas A&M University System Health Science Center, College Station, Texas 77843-1114,1 Centre for Molecular Microbiology and Infection, Imperial College of Science, Technology and Medicine, London SW7 2AZ, United Kingdom2

Received 12 July 2002/ Returned for modification 29 August 2002/ Accepted 25 October 2002

The shdA gene is carried on a 25-kb genetic island at centisome 54 (CS54 island) of the Salmonella enterica serotype Typhimurium chromosome. In addition to shdA, the CS54 island of Salmonella serotype Typhimurium strain LT2 contains four open reading frames designated ratA, ratB, sivI, and sivH. DNA hybridization analysis revealed that the CS54 island is comprised of two regions with distinct phylogenetic distribution within the genus Salmonella. Homologues of shdA and ratB were detected only in serotypes of Salmonella enterica subsp. I. In contrast, sequences hybridizing with ratA, sivI, and sivH were present in S. enterica subsp. II and S. bongori in addition to S. enterica subsp. I. Deletion of the ratA and sivI genes did not alter the ability of Salmonella serotype Typhimurium to colonize the organs of mice. Insertional inactivation of the sivH gene resulted in defective colonization of the Peyer's patches of the terminal ileum but normal colonization of the cecum, mesenteric lymph nodes, and spleen. Deletion of the shdA gene resulted in decreased colonization of the cecum and Peyer's patches of the terminal ileum and colonization to a lesser degree in the mesenteric lymph nodes and spleen 5 days post-oral inoculation of mice. A strain containing a deletion in the ratB gene exhibited a defect for the colonization of the cecum but not of the Peyer's patches, mesenteric lymph nodes, and spleen. The shdA and ratB deletion strains exhibited a shedding defect in mice, whereas the sivH deletion strain was shed at numbers similar to the wild type. These data suggest that colonization of the murine cecum is required for efficient fecal shedding in mice.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, College of Medicine, Texas A&M University System Health Science Center, Reynolds Medical Building, College Station, TX 77843-1114. Phone: (979) 862-7756. Fax: (979) 845-3479. E-mail: abaumler{at}tamu.edu.

Editor: A. D. O'Brien

Infection and Immunity, February 2003, p. 629-640, Vol. 71, No. 2
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.2.629-640.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Arvey, A. J., Azad, R. K., Raval, A., Lawrence, J. G. (2009). Detection of genomic islands via segmental genome heterogeneity. Nucleic Acids Res 37: 5255-5266 [Abstract] [Full Text]  
  • Haneda, T., Winter, S. E., Butler, B. P., Wilson, R. P., Tukel, C., Winter, M. G., Godinez, I., Tsolis, R. M., Baumler, A. J. (2009). The Capsule-Encoding viaB Locus Reduces Intestinal Inflammation by a Salmonella Pathogenicity Island 1-Independent Mechanism. Infect. Immun. 77: 2932-2942 [Abstract] [Full Text]  
  • Katribe, E., Bogomolnaya, L. M., Wingert, H., Andrews-Polymenis, H. (2009). Subspecies IIIa and IIIb Salmonellae Are Defective for Colonization of Murine Models of Salmonellosis Compared to Salmonella enterica subsp. I Serovar Typhimurium. J. Bacteriol. 191: 2843-2850 [Abstract] [Full Text]  
  • Klumpp, J., Fuchs, T. M. (2007). Identification of novel genes in genomic islands that contribute to Salmonella typhimurium replication in macrophages. Microbiology 153: 1207-1220 [Abstract] [Full Text]  
  • Abd El Ghany, M., Jansen, A., Clare, S., Hall, L., Pickard, D., Kingsley, R. A., Dougan, G. (2007). Candidate Live, Attenuated Salmonella enterica Serotype Typhimurium Vaccines with Reduced Fecal Shedding Are Immunogenic and Effective Oral Vaccines. Infect. Immun. 75: 1835-1842 [Abstract] [Full Text]  
  • Weening, E. H., Barker, J. D., Laarakker, M. C., Humphries, A. D., Tsolis, R. M., Baumler, A. J. (2005). The Salmonella enterica Serotype Typhimurium lpf, bcf, stb, stc, std, and sth Fimbrial Operons Are Required for Intestinal Persistence in Mice. Infect. Immun. 73: 3358-3366 [Abstract] [Full Text]  
  • Henderson, I. R., Navarro-Garcia, F., Desvaux, M., Fernandez, R. C., Ala'Aldeen, D. (2004). Type V Protein Secretion Pathway: the Autotransporter Story. Microbiol. Mol. Biol. Rev. 68: 692-744 [Abstract] [Full Text]  
  • Porwollik, S., Boyd, E. F., Choy, C., Cheng, P., Florea, L., Proctor, E., McClelland, M. (2004). Characterization of Salmonella enterica Subspecies I Genovars by Use of Microarrays. J. Bacteriol. 186: 5883-5898 [Abstract] [Full Text]  
  • Schouler, C., Koffmann, F., Amory, C., Leroy-Setrin, S., Moulin-Schouleur, M. (2004). Genomic subtraction for the identification of putative new virulence factors of an avian pathogenic Escherichia coli strain of O2 serogroup. Microbiology 150: 2973-2984 [Abstract] [Full Text]  
  • Kingsley, R. A., Abi Ghanem, D., Puebla-Osorio, N., Keestra, A. M., Berghman, L., Baumler, A. J. (2004). Fibronectin Binding to the Salmonella enterica Serotype Typhimurium ShdA Autotransporter Protein Is Inhibited by a Monoclonal Antibody Recognizing the A3 Repeat. J. Bacteriol. 186: 4931-4939 [Abstract] [Full Text]  
  • Stecher, B., Hapfelmeier, S., Muller, C., Kremer, M., Stallmach, T., Hardt, W.-D. (2004). Flagella and Chemotaxis Are Required for Efficient Induction of Salmonella enterica Serovar Typhimurium Colitis in Streptomycin-Pretreated Mice. Infect. Immun. 72: 4138-4150 [Abstract] [Full Text]  
  • Andrews-Polymenis, H. L., Rabsch, W., Porwollik, S., McClelland, M., Rosetti, C., Adams, L. G., Baumler, A. J. (2004). Host Restriction of Salmonella enterica Serotype Typhimurium Pigeon Isolates Does Not Correlate with Loss of Discrete Genes. J. Bacteriol. 186: 2619-2628 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»