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Infection and Immunity, February 2003, p. 671-675, Vol. 71, No. 2
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.2.671-675.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Restoration of Cytoadherence to an Adherence-Deficient Mutant of Mycoplasma arthritidis by Genetic Complementation

Leigh R. Washburn,1* Daniel W. Bird,1 and Kevin Dybvig2

Division of Basic Biomedical Sciences, University of South Dakota, Vermillion, South Dakota 57069,1 Department of Genomics and Pathobiology, University of Alabama at Birmingham, Birmingham, Alabama 352942

Received 6 September 2002/ Returned for modification 15 October 2002/ Accepted 27 October 2002

Mycoplasma arthritidis causes a severe septic arthritis in rats under natural and experimental conditions. An earlier study implicated a membrane lipoprotein designated MAA1 in cytadherence of M. arthritidis. In addition, a spontaneous adherence-deficient mutant was shown to contain a nonsense mutation in the gene encoding MAA1, resulting in production of a truncated product, MAA1{Delta}. In the present study, a wild-type maa1 gene carried on transposon Tn4001T was introduced into the low-adherence mutant by polyethylene glycol-mediated transformation. The presence of the tranposon and the wild-type maa1 gene in the chromosome of transformants was confirmed by PCR and Southern hybridization. The latter procedure also confirmed that each transformant contained a single copy of the transposon. Western immunoblotting showed that transformants produced both wild-type MAA1 and MAA1{Delta}, indicating that the introduced wild-type maa1 gene was functional. This phenotype was stably maintained after multiple subcultures even in the absence of antibiotic selection. Finally, transformants were shown to adhere to rat L-2 lung cells in culture at wild-type levels, providing confirmation for an important role for MAA1 in adherence.

* Corresponding author. Mailing address: Division of Basic Biomedical Sciences, University of South Dakota School of Medicine, 414 E. Clark St., Vermillion, SD 57069-2390. Phone: (605) 677-5170. Fax: (605) 677-6381. E-mail: lwashbur{at}usd.edu.

Editor: W. A. Petri, Jr.

Infection and Immunity, February 2003, p. 671-675, Vol. 71, No. 2
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.2.671-675.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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