This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Asai, Y.
Right arrow Articles by Ogawa, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Asai, Y.
Right arrow Articles by Ogawa, T.

 Previous Article  |  Next Article 

Infection and Immunity, February 2003, p. 717-725, Vol. 71, No. 2
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.2.717-725.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Oral Treponemes and Their Outer Membrane Extracts Activate Human Gingival Epithelial Cells through Toll-Like Receptor 2

Yasuyuki Asai, Takayoshi Jinno, and Tomohiko Ogawa*

Department of Oral Microbiology, Asahi University School of Dentistry, 1851-1 Hozumi, Motosu-gun, Gifu 501-0296, Japan

Received 25 July 2002/ Returned for modification 29 August 2002/ Accepted 3 November 2002

Oral treponemes are considered to be important in the development and progression of periodontal diseases. We investigated the mechanisms of recognition and activation of human gingival epithelial cells (HGEC) with the oral treponemes Treponema denticola, Treponema vincentii, and Treponema medium and their outer membrane extracts (OMEs). T. vincentii and T. medium but not T. denticola produced interleukin 8 (IL-8) in an HGEC culture. Further, all three treponemes induced IL-8 mRNA expression and NF-{kappa}B activation in HGEC. Among them, T. denticola especially exhibited trypsin- and chymotrypsin-like protease activities, and the addition of chymostatin, a chymotrypsin protease inhibitor, resulted in detectable IL-8 production by HGEC cultured with T. denticola. Additionally, IL-8 mRNA expression in HGEC cultured with the three treponemes and their OMEs was definitely inhibited by the mouse anti-human Toll-like receptor 2 (TLR2) monoclonal antibody TL2.1. These findings suggest that oral treponemes and their OMEs activate HGEC through TLR2.

* Corresponding author. Mailing address: Department of Oral Microbiology, Asahi University School of Dentistry, 1851-1 Hozumi, Hozumi-cho, Motosu-gun, Gifu 501-0296, Japan. Phone: 81-58-329-1421. Fax: 81-58-329-1421. E-mail: tomo527{at}dent.asahi-u.ac.jp.

Editor: T. R. Kozel

Infection and Immunity, February 2003, p. 717-725, Vol. 71, No. 2
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.2.717-725.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nussbaum, G., Ben-Adi, S., Genzler, T., Sela, M., Rosen, G. (2009). Involvement of Toll-Like Receptors 2 and 4 in the Innate Immune Response to Treponema denticola and Its Outer Sheath Components. Infect. Immun. 77: 3939-3947 [Abstract] [Full Text]  
  • Handfield, M., Baker, H.V., Lamont, R.J. (2008). Beyond Good and Evil in the Oral Cavity: Insights into Host-Microbe Relationships Derived from Transcriptional Profiling of Gingival Cells. JDR 87: 203-223 [Abstract] [Full Text]  
  • Ruby, J., Rehani, K., Martin, M. (2007). Treponema denticola Activates Mitogen-Activated Protein Kinase Signal Pathways through Toll-Like Receptor 2. Infect. Immun. 75: 5763-5768 [Abstract] [Full Text]  
  • Miyamoto, M., Ishihara, K., Okuda, K. (2006). The Treponema denticola Surface Protease Dentilisin Degrades Interleukin-1{beta} (IL-1{beta}), IL-6, and Tumor Necrosis Factor Alpha. Infect. Immun. 74: 2462-2467 [Abstract] [Full Text]  
  • Brozovic, S., Sahoo, R., Barve, S., Shiba, H., Uriarte, S., Blumberg, R. S., Kinane, D. F. (2006). Porphyromonas gingivalis enhances FasL expression via up-regulation of NF{kappa}B-mediated gene transcription and induces apoptotic cell death in human gingival epithelial cells.. Microbiology 152: 797-806 [Abstract] [Full Text]  
  • Lee, S.-H., Kim, K.-K., Rhyu, I.-C., Koh, S., Lee, D.-S., Choi, B.-K. (2006). Phenol/water extract of Treponema socranskii subsp. socranskii as an antagonist of Toll-like receptor 4 signalling. Microbiology 152: 535-546 [Abstract] [Full Text]  
  • Asai, Y., Hashimoto, M., Fletcher, H. M., Miyake, K., Akira, S., Ogawa, T. (2005). Lipopolysaccharide Preparation Extracted from Porphyromonas gingivalis Lipoprotein-Deficient Mutant Shows a Marked Decrease in Toll-Like Receptor 2-Mediated Signaling. Infect. Immun. 73: 2157-2163 [Abstract] [Full Text]  
  • Bian, X.-l., Wang, H.-t., Ning, Y., Lee, S. Y., Fenno, J. C. (2005). Mutagenesis of a Novel Gene in the prcA-prtP Protease Locus Affects Expression of Treponema denticola Membrane Complexes. Infect. Immun. 73: 1252-1255 [Abstract] [Full Text]  
  • Lee, S.-H., Kim, K.-K., Choi, B.-K. (2005). Upregulation of Intercellular Adhesion Molecule 1 and Proinflammatory Cytokines by the Major Surface Proteins of Treponema maltophilum and Treponema lecithinolyticum, the Phylogenetic Group IV Oral Spirochetes Associated with Periodontitis and Endodontic Infections. Infect. Immun. 73: 268-276 [Abstract] [Full Text]  
  • Hashimoto, M., Asai, Y., Ogawa, T. (2003). Treponemal Phospholipids Inhibit Innate Immune Responses Induced by Pathogen-associated Molecular Patterns. J. Biol. Chem. 278: 44205-44213 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»