Previous Article | Next Article 
Infection and Immunity, February 2003, p. 754-765, Vol. 71, No. 2
0019-9567/03/$08.00+0 DOI: 10.1128/IAI.71.2.754-765.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
Characterization of the Conformational Epitope of Guy's 13, a Monoclonal Antibody That Prevents Streptococcus mutans Colonization in Humans
Craig J. van Dolleweerd, Daniel Chargelegue, and Julian K.-C. Ma*
Department of Oral Medicine and Pathology at Guy's, King's, and St. Thomas's Hospital Medical Schools, London, United Kingdom
Received 21 March 2002/
Returned for modification 26 June 2002/
Accepted 9 October 2002
Guy's 13 is a mouse monoclonal antibody which recognizes streptococcal antigen I/II (SA I/II), a major cell surface glycoprotein of Streptococcus mutans. In a number of clinical trials, this antibody has been shown to prevent colonization in the human oral cavity. The aim of this study was to identify the SA I/II epitope recognized by Guy's 13. The data suggest that the epitope is conformational, delimited by two noncontiguous regions of the antigen: residues 45 to 457, within the N-terminal half of SA I/II, and residues 816 to 983, within the C-terminal half. In fluid-phase immunoassays a strict requirement for the simultaneous presence of both regions was demonstrated for antibody binding. Furthermore, these two regions of SA I/II were shown to have the ability to interact with each other in the absence of Guy's 13 antibody, suggesting that the normal conformation of SA I/II might be determined by the interaction of these two regions.
* Corresponding author. Mailing address: Department of Oral Medicine and Pathology, Floor 28 Guy's Tower, Guy's Hospital, London SE1 9RT, United Kingdom. Phone: 44 (020) 79555000, ext. 5459. Fax: 44 (020) 79554455. E-mail: julian.ma{at}kcl.ac.uk.
Editor: V. J. DiRita
Infection and Immunity, February 2003, p. 754-765, Vol. 71, No. 2
0019-9567/03/$08.00+0 DOI: 10.1128/IAI.71.2.754-765.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
-
Crowley, P. J., Seifert, T. B., Isoda, R., van Tilburg, M., Oli, M. W., Robinette, R. A., McArthur, W. P., Bleiweis, A. S., Brady, L. J.
(2008). Requirements for Surface Expression and Function of Adhesin P1 from Streptococcus mutans. Infect. Immun.
76: 2456-2468
[Abstract]
[Full Text]
-
Zhang, S., Green, N. M., Sitkiewicz, I., LeFebvre, R. B., Musser, J. M.
(2006). Identification and Characterization of an Antigen I/II Family Protein Produced by Group A Streptococcus. Infect. Immun.
74: 4200-4213
[Abstract]
[Full Text]
-
Oli, M. W., Rhodin, N., McArthur, W. P., Brady, L. J.
(2004). Redirecting the Humoral Immune Response against Streptococcus mutans Antigen P1 with Monoclonal Antibodies. Infect. Immun.
72: 6951-6960
[Abstract]
[Full Text]
-
Rhodin, N. R., Cutalo, J. M., Tomer, K. B., McArthur, W. P., Brady, L. J.
(2004). Characterization of the Streptococcus mutans P1 Epitope Recognized by Immunomodulatory Monoclonal Antibody 6-11A. Infect. Immun.
72: 4680-4688
[Abstract]
[Full Text]
-
Seifert, T. B., Bleiweis, A. S., Brady, L. J.
(2004). Contribution of the Alanine-Rich Region of Streptococcus mutans P1 to Antigenicity, Surface Expression, and Interaction with the Proline-Rich Repeat Domain. Infect. Immun.
72: 4699-4706
[Abstract]
[Full Text]
-
van Dolleweerd, C. J., Kelly, C. G., Chargelegue, D., Ma, J. K-C.
(2004). Peptide Mapping of a Novel Discontinuous Epitope of the Major Surface Adhesin from Streptococcus mutans. J. Biol. Chem.
279: 22198-22203
[Abstract]
[Full Text]
-
Rhodin, N. R., Van Tilburg, M. L. J. A., Oli, M. W., McArthur, W. P., Brady, L. J.
(2004). Further Characterization of Immunomodulation by a Monoclonal Antibody against Streptococcus mutans Antigen P1. Infect. Immun.
72: 13-21
[Abstract]
[Full Text]
Copyright © 2003 by the American Society for Microbiology. All rights reserved.