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Infection and Immunity, February 2003, p. 754-765, Vol. 71, No. 2
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.2.754-765.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Characterization of the Conformational Epitope of Guy's 13, a Monoclonal Antibody That Prevents Streptococcus mutans Colonization in Humans

Craig J. van Dolleweerd, Daniel Chargelegue, and Julian K.-C. Ma*

Department of Oral Medicine and Pathology at Guy's, King's, and St. Thomas's Hospital Medical Schools, London, United Kingdom

Received 21 March 2002/ Returned for modification 26 June 2002/ Accepted 9 October 2002

Guy's 13 is a mouse monoclonal antibody which recognizes streptococcal antigen I/II (SA I/II), a major cell surface glycoprotein of Streptococcus mutans. In a number of clinical trials, this antibody has been shown to prevent colonization in the human oral cavity. The aim of this study was to identify the SA I/II epitope recognized by Guy's 13. The data suggest that the epitope is conformational, delimited by two noncontiguous regions of the antigen: residues 45 to 457, within the N-terminal half of SA I/II, and residues 816 to 983, within the C-terminal half. In fluid-phase immunoassays a strict requirement for the simultaneous presence of both regions was demonstrated for antibody binding. Furthermore, these two regions of SA I/II were shown to have the ability to interact with each other in the absence of Guy's 13 antibody, suggesting that the normal conformation of SA I/II might be determined by the interaction of these two regions.

* Corresponding author. Mailing address: Department of Oral Medicine and Pathology, Floor 28 Guy's Tower, Guy's Hospital, London SE1 9RT, United Kingdom. Phone: 44 (020) 79555000, ext. 5459. Fax: 44 (020) 79554455. E-mail: julian.ma{at}kcl.ac.uk.

Editor: V. J. DiRita

Infection and Immunity, February 2003, p. 754-765, Vol. 71, No. 2
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.2.754-765.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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