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Infection and Immunity, February 2003, p. 754-765, Vol. 71, No. 2
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.2.754-765.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Characterization of the Conformational Epitope of Guy's 13, a Monoclonal Antibody That Prevents Streptococcus mutans Colonization in Humans

Department of Oral Medicine and Pathology at Guy's, King's, and St. Thomas's Hospital Medical Schools, London, United Kingdom

Received 21 March 2002/ Returned for modification 26 June 2002/ Accepted 9 October 2002

Guy's 13 is a mouse monoclonal antibody which recognizes streptococcal antigen I/II (SA I/II), a major cell surface glycoprotein of Streptococcus mutans. In a number of clinical trials, this antibody has been shown to prevent colonization in the human oral cavity. The aim of this study was to identify the SA I/II epitope recognized by Guy's 13. The data suggest that the epitope is conformational, delimited by two noncontiguous regions of the antigen: residues 45 to 457, within the N-terminal half of SA I/II, and residues 816 to 983, within the C-terminal half. In fluid-phase immunoassays a strict requirement for the simultaneous presence of both regions was demonstrated for antibody binding. Furthermore, these two regions of SA I/II were shown to have the ability to interact with each other in the absence of Guy's 13 antibody, suggesting that the normal conformation of SA I/II might be determined by the interaction of these two regions.

Editor: V. J. DiRita

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