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Infection and Immunity, February 2003, p. 904-909, Vol. 71, No. 2
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.2.904-909.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Colonial Morphology of Burkholderia cepacia Complex Genomovar III: Implications in Exopolysaccharide Production, Pilus Expression, and Persistence in the Mouse

Jacqueline W. Chung,1,2 Eleonora Altman,3,4 Terry J. Beveridge,4,5 and David P. Speert1,2,4*

Departments of Pathology and Laboratory Medicine,1 Pediatrics, University of British Columbia, Vancouver, British Columbia V5Z 4H4,2 Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario K1A 0R6,3 Department of Microbiology, University of Guelph, Guelph, Ontario N1G 2W1,5 Canadian Bacterial Disease Network, National Centre of Excellence, Calgary, Alberta T2N 4N1, Canada4

Received 22 July 2002/ Returned for modification 3 September 2002/ Accepted 31 October 2002

The purpose of this study was to determine the role of colonial morphology of Burkholderia cepacia complex (BCC) organisms in pathogenicity in a mouse model of pulmonary infection. BCC strain C1394 was rapidly cleared by leukopenic mice after intranasal challenge, whereas a spontaneous variant (C1394mp2) that was indistinguishable from the parent strain by genetic typing persisted in the lungs and differed in colonial morphology. The parent strain had a matte colonial phenotype, made scant exopolysaccharide (EPS), and was lightly piliated. The variant had a shiny phenotype, produced abundant EPS, and was heavily piliated. Matte to shiny colonial transformation was induced by growth at 42°C. Colonial morphology in the BCC strain variant was associated with persistence after pulmonary challenge and appeared to be correlated with the elaboration of putative virulence determinants.

* Corresponding author. Mailing address: B.C. Research Institute, Rm. 377, 950 West 28th Ave., Vancouver, British Columbia V5Z 4H4, Canada. Phone: (604) 875-2438. Fax: (604) 875-2226. E-mail: dspeert{at}cw.bc.ca.

Editor: D. L. Burns

Infection and Immunity, February 2003, p. 904-909, Vol. 71, No. 2
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.2.904-909.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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