This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Okahashi, N.
Right arrow Articles by Hamada, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Okahashi, N.
Right arrow Articles by Hamada, S.

 Previous Article  |  Next Article 

Infection and Immunity, February 2003, p. 948-955, Vol. 71, No. 2
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.2.948-955.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Infection by Streptococcus pyogenes Induces the Receptor Activator of NF-{kappa}B Ligand Expression in Mouse Osteoblastic Cells

Nobuo Okahashi,1,2* Atsuo Sakurai,1,3 Ichiro Nakagawa,1 Taku Fujiwara,3 Shigetada Kawabata,1,4 Atsuo Amano,2 and Shigeyuki Hamada1

Departments of Oral and Molecular Microbiology,1 Oral Frontier Biology,2 Pediatric Dentistry, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita-Osaka 565-0871,3 PRESTO, Japan Science and Technology Corporation, Kawaguchi-Saitama 332-0012, Japan4

Received 19 August 2002/ Returned for modification 10 October 2002/ Accepted 31 October 2002

Group A Streptococcus pyogenes is known to induce nongonococcal septic arthritis in addition to pharyngitis, scarlet fever, and poststreptococcal sequelae. However, little is known about the interaction between S. pyogenes and bone cells. We report here that S. pyogenes strain JRS4 (M6) attached to and invaded mouse primary osteoblasts. Reverse transcription-PCR demonstrated that S. pyogenes infection of osteoblasts stimulated expression of mRNA for the receptor activator of NF-{kappa}B ligand (RANKL). Western blot analysis followed by ligand precipitation with the receptor activator of NF-{kappa}B receptor showed that there was an increase in RANKL protein in infected osteoblasts. Production of interleukin-6 was also stimulated, but no production of interleukin-1ß or tumor necrosis factor alpha was observed. Stimulation of RANKL production was not observed in osteoblasts stimulated with heat-inactivated S. pyogenes, suggesting that an active interaction of S. pyogenes with osteoblasts is essential for this phenomenon. A Western blot analysis performed with antibodies specific for phosphorylated signal transduction proteins demonstrated that S. pyogenes infection induces phosphorylation of p38 mitogen-activated protein kinase. A specific inhibitor of this kinase, SB203580, inhibited RANKL production by infected osteoblasts. These results suggest that infection of osteoblasts by S. pyogenes stimulates RANKL production and may trigger bone destruction in infected bone tissue.

* Corresponding author. Mailing address: Department of Oral Frontier Biology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita-Osaka 565-0871, Japan. Phone: 81-6-6879-2976. Fax: 81-6-6879-2976. E-mail: okahashi{at}dent.osaka-u.ac.jp.

Editor: J. N. Weiser

Infection and Immunity, February 2003, p. 948-955, Vol. 71, No. 2
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.2.948-955.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Somayaji, S. N., Ritchie, S., Sahraei, M., Marriott, I., Hudson, M. C. (2008). Staphylococcus aureus Induces Expression of Receptor Activator of NF-{kappa}B Ligand and Prostaglandin E2 in Infected Murine Osteoblasts. Infect. Immun. 76: 5120-5126 [Abstract] [Full Text]  
  • Ishida, I., Kohda, C., Yanagawa, Y., Miyaoka, H., Shimamura, T. (2007). Epigallocatechin gallate suppresses expression of receptor activator of NF-{kappa}B ligand (RANKL) in Staphylococcus aureus infection in osteoblast-like NRG cells. J Med Microbiol 56: 1042-1046 [Abstract] [Full Text]  
  • Maruyama, K., Takada, Y., Ray, N., Kishimoto, Y., Penninger, J. M., Yasuda, H., Matsuo, K. (2006). Receptor Activator of NF-{kappa}B Ligand and Osteoprotegerin Regulate Proinflammatory Cytokine Production in Mice. J. Immunol. 177: 3799-3805 [Abstract] [Full Text]  
  • Ratner, A. J., Hippe, K. R., Aguilar, J. L., Bender, M. H., Nelson, A. L., Weiser, J. N. (2006). Epithelial Cells Are Sensitive Detectors of Bacterial Pore-forming Toxins. J. Biol. Chem. 281: 12994-12998 [Abstract] [Full Text]  
  • Okahashi, N., Inaba, H., Nakagawa, I., Yamamura, T., Kuboniwa, M., Nakayama, K., Hamada, S., Amano, A. (2004). Porphyromonas gingivalis Induces Receptor Activator of NF-{kappa}B Ligand Expression in Osteoblasts through the Activator Protein 1 Pathway. Infect. Immun. 72: 1706-1714 [Abstract] [Full Text]  
  • Sakurai, A., Okahashi, N., Nakagawa, I., Kawabata, S., Amano, A., Ooshima, T., Hamada, S. (2003). Streptococcus pyogenes Infection Induces Septic Arthritis with Increased Production of the Receptor Activator of the NF-{kappa}B Ligand. Infect. Immun. 71: 6019-6026 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»