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Infection and Immunity, February 2003, p. 991-996, Vol. 71, No. 2
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.2.991-996.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Epithelial Cell Surface Sites Involved in the Polyvalent Adherence of Porphyromonas gingivalis: a Convincing Role for Neuraminic Acid and Glucuronic Acid

G. Agnani, S. Tricot-Doleux, S. Houalet, and M. Bonnaure-Mallet*

Equipe de Biologie Buccale, UPRES-EA 1256, 35000 Rennes, France

Received 8 May 2002/ Returned for modification 3 July 2002/ Accepted 7 November 2002

We investigated the target structures of the epithelial cells responsible for the attachment of Porphyromonas gingivalis by immunocytofluorimetry, enzyme-linked immunosorbent assay, and confocal microscopy. Integrins (ß1, ß3, and {alpha}V) and E-cadherin played no significant role. Carbohydrates (such as {alpha}-D-methylglucoside, L-fucose, D- and L-mannose, N-acetylglucosamine, and N-acetylgalactosamine) had little inhibitory effect on bacterial binding. Enzymatic treatments of the epithelial membranes and sugar competition studies showed that N-acetylneuraminic acid and glucuronic acid were involved in binding.

* Corresponding author. Mailing address: Université de Rennes I, 2 Place Pasteur, 35000 Rennes, France. Phone: 33-2-23-23-43-58. Fax: 33-2-23-23-43-04. E-mail: martine.bonnaure{at}univ-rennes1.fr.

Editor: V. J. DiRita

Infection and Immunity, February 2003, p. 991-996, Vol. 71, No. 2
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.2.991-996.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Andrian, E., Grenier, D., Rouabhia, M. (2006). Porphyromonas gingivalis-Epithelial Cell Interactions in Periodontitis. JDR 85: 392-403 [Abstract] [Full Text]  
  • Hellstrom, U., Hallberg, E. C., Sandros, J., Rydberg, L., Backer, A. E. (2004). Carbohydrates act as receptors for the periodontitis-associated bacterium Porphyromonas gingivalis: a study of bacterial binding to glycolipids. Glycobiology 14: 511-519 [Abstract] [Full Text]  


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